HIF-1α、VEGF在妊娠期糖尿病胎盘组织中的表达及其与胎儿缺氧的关系  

Expression of Hypoxia-Inducible Factor-1α and Vascular Epithelial Growth Factor in Gestational Diabetic Placenta and Its Correlation with Fetal Hypoxia

作　　者：杨晓菊[1] 杨宗利[1] 王学芹[1] 戴淑真[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003

出　　处：《实用妇产科杂志》2012年第11期962-966,共5页Journal of Practical Obstetrics and Gynecology

基　　金：山东省青岛市科技局基金资助项目(编号:KZD-12)

摘　　要：目的:探讨缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)在妊娠期糖尿病(GDM)胎盘中的表达及其与血糖控制效果和胎儿缺氧的关系。方法:选取GDM孕妇64例,其中血糖控制不良组(A组)22例,血糖控制良好组(B组)42例,同期分娩正常孕妇27例为对照组(C组)。采用Giemsa染色法计数脐血中胎儿有核红细胞(FNRBC);免疫组化SP法和RT-PCR技术测定HIF-1α和VEGF蛋白和mRNA在胎盘中表达;分析GDM组FNRBC水平与HIF-1αmRNA及VEGFmRNA表达的相关性。结果:①3组胎盘中均有HIF-1α和VEGF蛋白的表达,A、B组胎盘中HIF-1α和VEGF蛋白的表达呈较强阳性和强阳性。②A、B、C组胎盘组织中HIF-1α和VEGF蛋白阳性表达率两两比较差异均有高度统计学意义(P<0.01)。③A、B、C组胎盘组织中HIF-1α及VEGFmRNA表达水平两两比较差异均有高度统计学意义(P<0.01)。④A、B、C组脐血FNRBC计数水平两两比较差异均有高度统计学意义(P<0.01)。⑤A、B组脐血FNRBC水平与胎盘组织中HIF-1α、VEGFmRNA表达均呈正相关关系(P<0.01);A、B组胎盘组织中HIF-1α与VEGFmRNA表达亦呈正相关关系(P<0.01)。结论:GDM胎盘中HIF-1α和VEGF的高表达可能参与了GDM的发病过程,其表达水平与孕妇血糖控制效果有关,且能导致胎儿缺氧和不良后果。Objective：To investigate the expression of hypoxia-inducible factor-1α（HIF-1α） and vascular epithelial growth factor（ VEGF） in gestational diabetic placenta and its correlation with glucose control effect and fetal hypoxia. Methods：A case-control study was performed in 64 women with gestational diabetes melli- tus（ GDM）, including 22 glucose poor-controlled （group A）women and 42 glucose well-controlled （group B） women,27 healthy pregnant women were taken as control group （group C）. Cord plasma FNRBC counts were detected by Giemsa staining; The expressions of HIF-1α and VEGF protein in placenta were detected by immunohistochemistry; The expressions of HIF-la and VEGF mRNA in placenta were detected by RT-PCR. The relationship between cord plasma FNRBC counts and HIF-1α mRNA and VEGF mRNA expres- sion in placenta was analyzed in the two GDM groups. Results：①HIF-1α and VEGF were expressed in the placenta of all pregnant women,while significantly higher in GDM groups. ②Statistical significance of HIF-1α and VEGF protein expression was found between any two of the three groups （ P〈0. 01 ）. ③Statistical sig- nificance of HIF-la and VEGF mRNA expression was found between any two of the three groups （ P 〈 0.01 ）. ④Statistical significance of cord plasma FNRBC counts was found between any two of the three groups （P〈0.01）.⑤In group A, B cord plasma FNRBC counts were positively correlated with the expres- sion of HIF-1α mRNA and VEGF mRNA （ P 〈 0.01 ）, and positive correlation was also found between the lev- els of HI F-1α and VEGF mRNA（ P 〈 0.01 ）. Conclusions： Overexpressions of HIF-1α and VEGF in GDMplacenta were correlated with blood glucose control and the cord plasma FNRBC counts,which might be in- volved in the fetal hypoxia.

关 键 词：妊娠期糖尿病 胎盘 缺氧诱导因子-1Α 血管内皮生长因子 胎儿有核红细胞 

分 类 号：R714.256[医药卫生—妇产科学]