妊娠期高血压和子痫前期患者胎盘及子宫浅肌层中转化生长因子-β1、endoglin蛋白及mRNA表达水平  

Expression of endoglin and transforming growth factor-β1 in placenta and superficial myometrium ofwomen with gestational hypertension or preeclampsia

作　　者：张俊农[1] 许群星[1] 韩玉环[1] 张立军[1] 华绍芳[1] 董微[1] 

机构地区：[1]天津医科大学第二医院产科,300211

出　　处：《中华围产医学杂志》2012年第12期732-736,共5页Chinese Journal of Perinatal Medicine

基　　金：天津市自然科学基金（09JCYBJC13000）

摘　　要：目的研究妊娠期高血压和子痫前期患者胎盘组织和子宫浅肌层中转化生长因子-β1（transforming growth factor-β1，TGF-β1）、endoglin（Eng）蛋白及mRNA表达水平及其相关性，探讨Eng与TGF-β1在妊娠期高血压和子痫前期发病中的作用。方法选取2009年4月至2010年4月在天津医科大学第二医院产科住院并分娩的孕妇共110例，其中妊娠期高血压组30例，轻度子痫前期组30例，重度子痫前期组30例，正常对照组20例。剖宫产时取胎盘及子宫浅肌层组织。采用Western印迹技术测定胎盘及子宫浅肌层中Eng、TGF-β1蛋白的表达；实时荧光定量聚合酶链反应技术检测各组孕妇胎盘及子宫浅肌层中Eng及TGF—βmRNA表达情况。多组比较采用单因素方差分析，两两比较采用Student-Newman-Keuls检验；相关性用Pearson相关分析及直线回归分析。结果在正常对照组、妊娠期高血压组、轻度子痫前期组和重度子痫前期组，胎盘组织Eng蛋白表达水平分别为0．11±0．07、0．15±0．05、0．18±0．06和0．43±0．04，TGFB1蛋白表达水平分别为0．11±0．02、0．26±0．05、0．27±0．03和0．88±0．09；子宫浅肌层中Eng蛋白表达水平分别为0．14±0．06、0．16±0．04、0．20±0．08和0．46±0．05，TGF-β1蛋白表达水平分别为0．15±0．03、0．29±0．06、0．31±0．04和0．91±0．08。在正常对照组、妊娠期高血压组、轻度子痫前期组和重度子痫前期组，胎盘组织EngmRNA表达水平分别为1．00、1．27±0．58、1．54±0．41和1．83±0．35，TGF-β1mRNA表达水平分别为1．00、1．64±0．33、1．92±0．38和2．23±0．53；子宫浅肌层中EngmRNA表达水平分别为1．00、1．32±0．46、1．59±0．37和1．93±0．52，TGF-β1mRNA表达水平分别为1．00、1．71±0．45、1．91±0．51和2．37±0．46。随着病情的加重，2种因子蛋白和mRNA的表达均呈上升趋势，组间差异均有统计学意义（P均�Objective To investigate the expression of transforming growth facto-β1 （TGF-β1）, endoglin （Eng） and their mRNA in placenta and superficial myometrium of patients with gestational hypertension or preeclampsia and to explore the role of Eng and TGF-β1 in the pathogenesis of gestational hypertension or preeclampsia. Methods One hundred and ten pregnant women were selected in the Second Affiliated Hospital of Tianjin Medical University from April 2009 to April 2010 who underwent cesarean sections and were divided into four groups： gestational hypertension group （n=30）, mild preeclampsia group （n=30）, severe preeclampsia group （n=30） and control group （normal pregnant women without labor and perinatal complications, n=20）. The tissues of placenta and superficial myometrium were collected during cesarean section. Protein levels of Eng and TGF-β1 were detected by Western Blot. Real-time fluorescence reverse transcription polymerase chain reaction was used to detect the Eng and TGF-β1 mRNA expression. One-way ANOVA was used to compare among groups, Student-Newman-Keuls test was used to compare the differences between groups; relation between groups was analyzed by Pearson relation and linear regression. Results In control group, gestational hypertension group, mild and severe preeclampsia group, the Eng mRNA level was 1.00, 1.27±0.58, 1.54±0.41 and 1.83±0.35, and the TGF-β1 mRNA level was 1.00, 1.64 ±0.33, 1.92 ± 0.38 and 2.23 ± 0.53 in placenta respectively; those figures changed to 1.00, 1.32±0.46, 1.59±0.37 and 1.93±0.52, and 1.00, 1.71 ± 0.45, 1.91 4 0.51 and 2.37 ± 0.46 in superficial myometrium respectively. The Eng and TGF-β1 mRNA levels of control group were lower than those of the other three groups （P〈0.05）. The higher the mRNA level, the more severe the disease （P〈0.05）. In control group,gestational hypertension group, mild and severe preeclampsia group, the Eng protein expression was 0.11±0.07, 0.15± 0.05, 0.18±0.06 and 0.43 ± 0.04, and the TGF-β1

关 键 词：高血压 妊娠性 胎盘 子宫肌层 转化生长因子β1 抗原 CD 受体 细胞表面 

分 类 号：R714.2[医药卫生—妇产科学]