白细胞介素-17对巨噬细胞分化的影响及在闭塞性细支气管炎中的作用  

Interleukin 17 decrease the polarization of macrophage and attenuate the pathology of obliterative bronchiolitis aftertrachea transplant modulated

作　　者：王建刚[1] 周晓慧[1] 倪寅凯[1] 史乾[1] 曹浩[1] 范慧敏[1] 

机构地区：[1]同济大学附属东方医院心外科,上海200120

出　　处：《中华胸心血管外科杂志》2012年第12期732-734,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金（81070073）;上海市卫生局科研课题计划（20114231）;浦东新区卫生系统领先人才培养（PWRl2011-011

摘　　要：目的探讨白细胞介素-17（IL-17）对巨噬细胞分化的影响，及其在小鼠闭塞性细支气管炎发展中的作用。方法选取20-25g清洁级雄性小鼠。C57BL／6，20只；IL-17基因敲除C57BL／6，10只；BALB／c，10只；按体质量随机配对分为3组。实验组A：BALB／c（5只）→C57BL／6（10只）；实验组B：BALB／c（5只）-IL-17基因敲除C57BL／6（10只）；对照组：正常C57BL／6（10只）未手术组。于术后第7天检测脾脏中巨噬细胞的比例和CD80的表达水平，于第14天、28天取出移植气管分别进行病理形态学检测。结果与对照组相比，实验组A巨噬细胞比例均显著增高；实验组B巨噬细胞比例相对于实验组A明显降低。第14天、28天实验组B的气管上皮损伤及纤维化程度相对于实验组A显著减轻。结论IL-17基因敲除明显下调气管移植小鼠体内巨噬细胞表面CD80的表达水平，并明显减轻气管移植物的病理改变。Objective To investigate the role of interleukin 17 in the differentiation of macrophages from monocyte in vi- vo and its applicability in Obliterative bronchiolitis （OB）. Methods Pathogen-free male C57BL/6 （n = 20） , IL-17-defcient C57BL/6 （n = 10） and BALB/c （n = 10） weighing 20 -25 g. Weight-matched mice were assigned to three experimental groups. Experimental group A ： BALB/c （ n = 5 ）→C57BL/6 （ n = 10 ）. Experimental group B ： BALB/c （ n = 5 ） →IL-17-def- cient C57BL/6 （n = 10）. Normal control group： C57BL/6 （n = 10）. The CD80 expression in macrophages in spleens were ana- lysed by flowcytometry 7 days after operation. Native and transplanted lungs were harvested after 14 days and 28 days, stained with HE, and examined under light microscopy. Results Compared to experimental group A, CD80 expression of macropha- ges reveales a significant decrease in spleen of experimental group B. Furthermore, airway obliteration and destruction of the epithelium in experimental group A were significantly better compared with that of experimental group B on day 14 and 28 after transplantation. Conclusion IL-17 deficiency decreased the polarization of monocytes to macrophages and attenuate the pa- thology of obliteral bronchiolitis in murine model after trachea transplantaion.

关 键 词：移植 巨噬细胞 细支气管炎 闭塞性 模型 动物 白细胞介素17 

分 类 号：R655.3[医药卫生—外科学]