产油微藻筛选和鉴定及其产油性能的研究  

Study on Screening and Identification of Oleaginous Microalgae and Its Oil-producing Charateristic

作　　者：胡文军[1] 罗玮[1] 李汉广[1] 顾秋亚[1] 余晓斌[1] 

机构地区：[1]江南大学生物工程学院糖化学与生物技术教育部重点实验室,无锡214122

出　　处：《中国生物工程杂志》2012年第12期66-72,共7页China Biotechnology

基　　金：国家自然科学基金(21176105);江苏省优势学科工程资助项目

摘　　要：为了筛选具有产油能力的微藻,从自然界水体中分离出14株微藻,根据形态特点对它们进行了初步鉴定。对其中12株微藻在自养和异养条件下的生长特性和产油性能进行了比较。通过微藻的生长曲线,生物量和油脂含量等指标,从中筛选出高产藻株并对该藻株进行了分子生物学鉴定。结果表明:藻株Y06在12种微藻中的油脂产量和产率最高,经18S rDNA鉴定确定为栅藻(Scenedesmus abundans)。藻株Y06在自养条件下的油脂产率为9.40 mg/(L.d),在异养条件下的油脂产率为201.29 mg/(L.d)。In order to screen microalgae that has the potential of oil production, fourteen strains of microalgae were isolated from the natural water and preliminarily identified by their morphological characteristics. Growth characteristics and lipid production capacity of twelve strains of microalgae were compared under autotrophic and heterotrophic conditions. High-yield strains were identified by molecular bioteehnology after being screened by the growth curve of microalgae, biomass and fat content and other indicators. The following results were obtained： Y06, one of the 12 species of microalgae, which was identified as Scenedesmus abundans by 18S rDNA analysis, has the highest oil production and productivity. The lipid productivity of Y06 were 9.40mg/L. d and 201.29 mg/L. d under autotrophic and heterotrophic conditions, respectively.

关 键 词：微藻 生物柴油 筛选鉴定 生物量 油脂产率 

分 类 号：Q949.93[生物学—植物学]