分子拥挤条件下生物大分子性质研究  

Studies on the Properties of Biomacromolecules under Molecular Crowded Conditions

作　　者：张玉姣[1] 唐乾[1] 曹洪玉[1] 郑学仿[2] 

机构地区：[1]大连大学生命科学与技术学院,大连116622 [2]辽宁省生物有机化学重点实验室,大连116622

出　　处：《中国生物工程杂志》2012年第12期104-109,共6页China Biotechnology

基　　金：国家自然科学基金(21271036;20871024);辽宁省高校创新团队项目(2010T002)资助项目

摘　　要：细胞中成千上万种生物分子以不同的浓度发挥着各自的生理作用,但是体外研究中常常忽略细胞内的拥挤环境,随着分子拥挤理论的提出,体外添加大分子拥挤试剂被越来越多的生物学家和化学家所重视,众多的研究结果显示分子拥挤试剂的加入对细胞内的生物大分子的性质造成了一定的影响。从分子拥挤试剂对蛋白质折叠与聚集以及核酸分子结构与性质的影响等方面探讨了拥挤环境下生物大分子的性质和功能,为以后大分子拥挤条件下生物分子的研究提供一定的参考依据。Living cells contain a variety of biomolecules, they play their physiological functions in different concentrations, which is often neglected in vitro studies. With the molecular crowding theory put forward, the addition of crowding agents in vitro is valued by more and more biologists and chemists, many research results show that the molecular crowding has effects on the properties of the biomolecules. The properties and functions of biomolecules from the effects of molecular crowding on protein folding, aggregation, enzymatic activity, the nucleic acids molecular structure and properties under the crowding conditions were discussed, to provide more references for the further research.

关 键 词：拥挤环境 生物大分子 蛋白质 核酸 

分 类 号：Q64[生物学—生物物理学] Q71