氨氯地平下调内皮细胞源性PDGF-B表达抑制平滑肌细胞增殖和迁移  

Amolodipine Inhibites the Cell Proliferation and Migration of HUASMC by Down-regulating Platelet-derived Growth Factor-B Expression Derived from HUVEC-12 Cells

作　　者：何谨[1] 孟军[1] 白洁[1] 涂剑[2] 许选选 王佐[3] 涂玉林[3] 

机构地区：[1]南华大学附属第一医院,湖南省衡阳市421001 [2]南华大学药物药理研究所,湖南省衡阳市421001 [3]南华大学医学院,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2012年第12期1093-1096,共4页Chinese Journal of Arteriosclerosis

基　　金：湖南省卫生厅科技项目(B2007101);博士启动基金(B2011XQD44)资助

摘　　要：目的观察人脐静脉内皮细胞12(HUVEC-12)受氧化型低密度脂蛋白刺激及氨氯地平作用后,血小板源生长因子B(PDGF-B)的内源性表达改变对人脐动脉平滑肌细胞(HUASMC)增殖和迁移的影响。方法酶联免疫吸附法检测不同浓度氨氯地平预孵育对氧化型低密度脂蛋白诱导的HUVEC-12细胞培养上清液中PDGF-B蛋白的表达。选择10.0μmol/L氨氯地平和20μmol/L PDGF-B抗体分别预孵育HUASMC 30 min,然后与PDGF-B上清液共同孵育24 h,噻唑蓝法检测不同细胞增殖能力的变化,Transwell迁移实验检测细胞迁移能力的改变。结果氨氯地平可下调氧化型低密度脂蛋白诱导的HUVEC-12细胞培养上清液中PDGF-B的蛋白表达,并能使预孵育HUASMC组细胞的增殖和迁移能力明显降低。结论氨氯地平下调内皮细胞源性PDGF-B的表达可抑制HUA-SMC的增殖和迁移。Aim To investigate the effect of platelet-derived growth factor-B （PDGF-B） expression derived from human umbilical vein endothelial cell-12 （HUVEC-12） on the cell migration of human umbilical arterial smooth muscle cells （HUASMC）. Methods HUVEC-12 cells were pretreated with different concentrations of amlodipine （0, 0. 1, 1.0, 10. 0 μmoL/L） for 30 min and then incubated with 50 mg/L oxidized low density lipoprotein （ox-LDL） for 24 h. PDGF-B protein expression was detected by enzyme-linked immunosorbent assay （ELISA） in the culture medium. HUA- SMC were pretreated with 10. 0 μmol/L amlodipine or 20μmol/L PDGF-B antibody for 30 min respectively and then incu- bated with PDGF-B suspernatant fluid for 24 h, the cell proliferation was determined by thiazolyl blue （MTT） and the mi- gration was determined by Transwell migration experiment. Results Amlodipine could down-regulate the PDGF-B pro- tein expression in HUVEC-12 cells induced by ox-LDL. The cell proliferation and migration were both significantly de- creased in the amlodipine pretreated group, the same as PDGF-B antibody pretreated group. Conclusion Amlodipine inhibits HUASMC proliferation and migration by down-regulating endothelial cell-derived PDGF-B expression.

关 键 词：氨氯地平 人脐静脉内皮细胞 血小板源性生长因子B 人脐动脉平滑肌细胞 细胞增殖和 迁移 

分 类 号：R285.5[医药卫生—中药学]