钙对大鼠下丘脑-垂体-性腺轴功能的影响  

Effects of calcium on hypothalamus-pituitary-gonadal axis in female rats

作　　者：王旭[1] 朱子阳[1] 石星[1] 

机构地区：[1]南京医科大学附属南京儿童医院内分泌病科,江苏省210008

出　　处：《江苏医药》2012年第24期2920-2922,共3页Jiangsu Medical Journal

基　　金：南京市医学科技发展基金(QYK10162)

摘　　要：目的探讨钙对下丘脑-垂体-性腺轴的影响。方法 20日龄雌性SD大鼠50只随机均分为正常对照组(每日生理盐水灌胃,6d后生理盐水0.2ml皮下注射,每日2次)、性早熟模型组(每日生理盐水灌胃,6d后N-甲基-DL-天冬氨酸40mg/kg皮下注射,每日2次)和钙高、中、低剂量组(分别用碳酸钙0.5、0.25、0.125g·kg-1·d-1灌胃,6d后生理盐水0.2ml皮下注射,每日2次)。计算大鼠子宫指数、卵巢指数,实时荧光定量PCR法检测下丘脑促性腺激素释放激素(GnRH)mRNA的表达。结果钙高剂量组子宫指数、卵巢指数及下丘脑GnRH mRNA含量与性早熟模型组相仿(P>0.05),均高于钙中、低剂量组和正常对照组(P<0.05);钙中、低剂量组上述指标与正常对照组相仿(P>0.05)。结论钙与大鼠下丘脑-垂体-性腺轴密切相关,对性发育起重要调节作用。Objective To investigate the effects of calcium on hypothalamus-pituitary-gonadal axis in female rats. Methods Fifty 20-day-old female SD rats were equally randomized into five groups of C （given normal saline by gavage per day and subcutaneously injected with normal saline 0. 2 ml twice a day after 6 days）, P （given normal saline by gavage per day and subcutaneously injected with N-methyl-DL-aspartate 40 mg/kg twice a day after 6 days）, H,M and L （given calcium carbonate by gavage in three doses of 0. 5,0. 25 and 0. 125 g · kg-1 · d-1 , respectively, and subcutaneously injected with of normal saline 0. 2 ml twice a day after 6 days）. The uterus and ovary indexes were calculated and the expression of hypothalamic gonadotropin-releasing hormone（GnRH） mRNA was detected by RT-PCR. Results Compared with groups of M, L and C, the uterus and ovary indexes and the expression of hypothalamic GnRH mRNA were higher in groups of H and P（P〈0. 05）, which showed no significant differences among the groups of M,L and C（P〉0. 05）. Conclusion Calcium is closely related with hypothalamus-pituitary-gonadal axis, which may play an important role in the regulation of sexual development.

关 键 词：钙 下丘脑-垂体-性腺轴 促性腺激素释放激素 

分 类 号：R585[医药卫生—内分泌]