丙泊酚对大鼠肝脏I-R损伤性胰岛β细胞功能和外周组织胰岛素敏感性下降的影响  

Effects of propofol on decreased secretion of pancreatic beta cells and the insulin sensitivity caused by hepatic ischemia-reperfusion injury in rats

作　　者：吴瑶[1] 刘存明[2] 徐鑫[1] 马正良[1] 

机构地区：[1]南京大学附属鼓楼医院麻醉科,江苏省210008 [2]南京医科大学附属第一医院麻醉科

出　　处：《江苏医药》2012年第24期2926-2928,共3页Jiangsu Medical Journal

摘　　要：目的观察丙泊酚对大鼠肝脏缺血-再灌注(I-R)损伤性胰岛β细胞分泌功能和外周组织胰岛素敏感性下降的影响。方法 40只SD大鼠随机均分为丙泊酚组(P组)和I-R组。建立70%肝脏缺血1h、再灌注12h大鼠模型后,P组从肝门阻断前20min泵入丙泊酚20mg·kg-1·h-1直至再灌注2h,I-R组泵入等量生理盐水。再灌注12h后每组取10只采血,测定血糖、胰岛素浓度,并计算胰岛素分泌指数;每组另取10只行高血糖钳夹实验,测定血胰岛素浓度,计算葡萄糖代谢率和胰岛素敏感性指数。结果与I-R组相比,P组血糖和胰岛素浓度降低[(11.65±1.02)mmol/L vs. (6.23±3.67)mmol/L和(116.45±10.87)mU/L vs. (90.24±5.68)mU/L](P<0.05或P<0.01),胰岛素分泌指数升高(473.75±46.24 vs. 857.63±20.56)(P<0.01)。高血糖钳夹实验显示,与I-R组相比,P组血胰岛素浓度、胰岛素敏感性指数、葡萄糖代谢率升高[(69.05±13.10)mU/L vs. (84.27±15.04)mU/L、32.33±7.02 vs. 58.31±7.69、(29.62±7.43)mg·kg-1·min-1 vs. (56.09±10.56)mg·kg-1·min-1](P<0.05或P<0.01)。结论丙泊酚能缓解大鼠肝脏I-R损伤导致的胰岛β细胞分泌功能和外周组织胰岛素敏感性的下降。Objective To observe the effects of propofol on decreased secretion of pancreatic beta cells and the insulin sensitivity caused by ischemia-reperfusion（I-R） injury in rats. Methods The hepatic I-R injury model was established in 40 SD rats by 70% hepatic ischemia for I h and reperfusion for 12 h. The rats were equally randomized into two groups of P （treated with propofol via jugular vein from 20 min before ischemia to 120 min after reperfusion） and I-R （treated with equal volume of normal saline）. The concentrations of blood glucose and plasma insulin were determined and insulin secretion index was calculated at 12 h after reperfusion in 10 rats of each group. Hyperglycemia clamp test was performed at 12 h after reperfusion in other 10 rats of each group. The concentration of plasma insulin was determined and insulin sensitivity index and glucose metabolism rate were calculated. Results Compared with group I-R, the concentrations of blood glucose and plasma insulin were drcreased [（11.65±1.02） mmol/L vs. （6. 23±3.67） mmol/L and （116.45±10.87） mU/L vs. （90. 24 ± 5.68） mU/L, respectively] （P〈0. 05 or P〈0. 01 ）, while insulin secretion index was increased（473.75±46.24 vs. 857.63±20. 56）（P〈0. 01） in group P. The hyperglycemia clamp test showed that the concentration of plasma insulin, insulin sensitivity index and glucose metabolism rate in group P were higher than those in group I-R [（84.27±15. 04） mU/L vs. （69. 05±13. 10） mU/L, 58.3±7.69 vs. 32.33±7.02 and （56.09±10.56） mg·kg-1.min-1 vs. （29.62±7.43） mg·kg-1· min1 ,respectively] （P〈0. 05 or P〈0. 01）. Conclusion Propofol can attenuate the decreases of insulin secretion and sensitivity caused by hepatic I-R injury in rats.

关 键 词：丙泊酚 肝脏缺血-再灌注损伤 胰岛素抵抗 

分 类 号：R587[医药卫生—内分泌]