低温异养硝化菌的鉴定及对小白鼠的安全性研究  

Study on Identify of Low Temperature Heterotrophic Nitvification Bacteria and Safety of ICR Rats

作　　者：黄晓飞[1] 李伟光[1,2] 张多英[3] 潘忠诚[1] 秦雯[1] 宿程远[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,哈尔滨150001 [2]城市水资源国家研究中心,哈尔滨150001 [3]黑龙江东方学院,哈尔滨150001

出　　处：《科技通报》2012年第12期24-26,共3页Bulletin of Science and Technology

基　　金：国家自然科学基金项目(51078106)

摘　　要：对一株在低温环境中具有较强硝化能力的异养硝化菌经菌落特征观察、革兰氏染色镜检和生化特性进行研究,鉴定该菌为不动杆菌属(Acinetobacter.)成员.同时通过持续饲喂法和腹腔注射法对小白鼠进行安全性试验,结果:通过腹腔注射途径确定的半数致死量LD(50)为5.41×1011CFU/kg;经口服途径持续饲喂3个月低温异养硝化菌(Acinetobacter.Y20)1.5×1012CFU/kg体重以下是安全的,从而表明该菌对哺乳动物是安全的。In this paper, a strain in low temperature environment with strong nitrification capacity by the colony charac- teristics of heterotrophic nitrification bacteria, Gram staining and biochemical characteristics were studied, the identifica- tion ofthe bacterium Acinetobacter species （ Acinetobacter. ） members. At the＇ same time through continuous feeding method and intraperitoneal injection on mice for safety tests, results： through intraperitoneal injection of pathways that de- termine the half lethal dose of LD （50） to 5.41 x 1011CFU/kg; by the oral route continued feeding of low temperature for 3 months of heterotrophic nitrification bacteria （ Acinetobaeter.Y20 ） 1.5 x 1012CFU/kg body weight following is safe, thereby indicating that the bacteria to mammals is safe.

关 键 词：低温 异养硝化菌 ICR小白鼠 安全性 

分 类 号：Q494[生物学—生理学]