从高温高压蒸煮过的中华鳖甲和骨骼中提取及扩增DNA  

Extraction and Amplification of DNA from Autoclaved Chinese Soft Shelled Turtle (Trionyx sinensis) Shell and Bone

作　　者：刘中权[1] 王义权[1] 周开亚[1] 

机构地区：[1]南京师范大学遗传资源研究所,210097

出　　处：《中草药》2000年第5期343-345,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金!( No.3 9870 913 );江苏省教委科学基金!( 98KJB3 60 0 10 );江苏省"3 3 3工程"人才培养基金

摘　　要：为验证经高温加工过的鳖甲是否可以用 DNA分子标记鉴定 ,选取经高温高压蒸煮过的中华鳖鳖甲和骨进行 DNA提取 ,并用通用引物 L 10 91和 H1478扩增。结果表明经高温高压蒸煮过的鳖甲也可以提得较高质量的DNA,与从肌肉和药材鳖甲中提取的 DNA比较没有明显差异 ,并可用作模板进行 PCR扩增 ,得到约 40 0 bp基因片段 ;同时还讨论了 DNA提取和扩增过程中应注意的问题 ,为分子遗传标记技术广泛应用于同类药材的鉴定积累了基础资料。In order to ascertain whether autoclaved Trionyx sinensis Wiegmann could be authenticated by DNA molecular technology, DNA from boiled, steamed, and autoclaved shells and bones of the turtle were extracted and the template DNA amplified by universal primers L 1091 and H 1478. The results showed that there were no differences between the DNA obtained from the autoclaved material in comparison with those obtained from the boiled, steamed or traditionally processed crude drug. A 400 bp DNA fragment was amplified successfully from both boiled bone and muscles of crude drug under standard PCR conditions using the universal primers. The issue of DNA extraction and amplification were also discussed.

关 键 词：中华鳖 骨骼 DNA提取 PCR扩增 

分 类 号：R282.74[医药卫生—中药学]