多柔比星磁性蛋白微球的研制及体内外抗癌作用的实验研究  

Preparation of Daunorbicin Magnetic Albumin Microspheres and Their Experimental Antitumor Effects in vitro and in vivo

作　　者：陶凯雄[1] 陈道达[1] 田源[1] 王国斌[1] 吴在德[2] 

机构地区：[1]同济医科大学附属协和医院普外科,湖北武汉430022 [2]同济医科大学附属同济医院普外科,湖北武汉430030

出　　处：《中国医药工业杂志》2000年第5期210-213,共4页Chinese Journal of Pharmaceuticals

基　　金："九五"国家重点医学科技攻关专题资助项目!( 9690 60 116)

摘　　要：利用加热固化蛋白质原理制备多柔比星 (1)磁性蛋白微球 (2 )并检测其理化性质 ,以改良 MTT法分析其体外细胞毒作用 ,观察经消化道靶向给药后对鼠移植性胃肿瘤的治疗作用。结果表明 :2具有磁性药物微球的形态特征 ,体外对恶性肿瘤细胞的杀伤作用与游离 1相似 (P>0 .0 5 ) ,与恒定磁场联合应用对鼠移植性胃肿瘤生长的体内抑制明显高于单纯 1组 (P<0 .0 1)。因而 2是抗癌药物的一种新型制剂 。The daunorubicin magnetic albumin microspheres (ADM MAM) as a new preparation of daunorubicin were prepared by the protein heat coagulation method. Their physico chemical characteristics were examined. ADM MAM showed the same in vitro cytotoxic activities to the malignant tumor cell as those of free daunorubicin ( P >0.05), assayed by a modified MTT method. Meanwhile their inhibitory effects to growth of the implanted gastric tumor in Wistar rats were obviously increased vs . the control group ( P <0.01), administrated through alimentary canal in the presence of the external magnetic fields.\;

关 键 词：多柔比星 磁性微球 细胞毒 体内外抗癌作用 

分 类 号：R979.1[医药卫生—药品] R944.9[医药卫生—药学]