胰岛素产生细胞的诱导分化及微囊对其分泌能力的影响  

Effects of induced differentiation and micro-capsule on the secretion capacity of insulin-producing cells

作　　者：王雅光[1] 田鹤[1] 刘建生[2] 穆长征[1] 

机构地区：[1]辽宁医学院基础学院组织学与胚胎学教研室,辽宁锦州121001 [2]辽宁医学院基础学院解剖学教研室,辽宁锦州121001

出　　处：《西安交通大学学报（医学版）》2013年第1期38-41,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：辽宁省自然科学基金资助项目(No.201102138)~~

摘　　要：目的探讨微囊对胰岛素产生细胞(insulin-producing cells,IPCs)分泌能力的影响。方法分离培养小鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSCs)并传代纯化,应用大鼠胰腺提取物(rat pancreatic extract,RPE)对其进行诱导分化,诱导后的细胞随机分为微囊化组和未微囊化组。分别在1、2、3、4、5周等不同时间点,通过葡萄糖刺激实验,检测细胞的胰岛素与C肽释放情况。结果葡萄糖刺激实验结果显示,1周时微囊化IPCs与未微囊化的IPCs均能释放胰岛素,其量没有显著的差别;培养2周后未微囊化的IPCs胰岛素和C肽释放量开始下降,而微囊化IPCs胰岛素与C肽释放量在培养4周后才开始逐渐下降。结论微囊可延长IPCs的作用时间,有利于IPCs作用的发挥。Objective To study the effects of micro-capsule on the secretion capacity of insulin-producing cells （IPCs）. Methods Mouse bone marrow mesenchymal stem ceils （BMMSCs） were isolated, induced and purified. Rat pancreatic extract （RPE） was extracted from rats＇ pancreases. BMMSCs were induced by RPE. The induced cells were randomly divided into micro-encapsulated group and non-micro-encapsulated group. The experiment of glucose stimulation was performed to detect the level of insulin and C peptide release, respectively, at different time points （1 week, 2 week, 3 week, 4 week and 5 week）. Results The experiment of glucose stimulation showed that the level of insulin secretion did not differ in micro-encapsulated IPCs and non-micro-encapsulated IPCs at 1 week. The levels of insulin and C peptide secretion started to decline in non-micro-encapsulated IPCs at 2 week, while those did not decline in micro-encapsulated IPCs until 4 week. Oonclusion The micro-capsule can extend the effect duration of IPCs, which supports the function of IPCs.

关 键 词：微囊 胰岛素产生细胞 骨髓间充质干细胞 诱导分化 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]