转化生长因子-β_1在呼吸机相关肺损伤中的作用  

Effect of transforming growth factor-β_1 in ventilator induced lung injury

作　　者：刘伯毅[1] 郑翔[1] 刘培[1] 方志成[1] 黄雨[1] 

机构地区：[1]湖北医药学院附属太和医院重症医学科,湖北十堰442000

出　　处：《安徽医药》2012年第12期1749-1751,共3页Anhui Medical and Pharmaceutical Journal

基　　金：湖北省十堰市科技攻关项目(No2009045D)

摘　　要：目的观察转化生长因子-β1(Transforming Growth Factor-β1,TGF-β1)在呼吸机相关性肺损伤(Ventilator Induced Lung In-jury,VILI)中在作用,探讨VILI发生的机制。方法将40只健康雄性Wistar大鼠随机分为对照组(插管后不进行机械通气)及实验组(插管后机械通气:潮气量20 ml·kg-1,频率为每分钟50次,吸呼比为1∶3,FiO2为21%,通气时间为5 h),观察实验前后动脉氧分压变化;取左肺中央部分肺组织,制备肺组织匀浆,采用链霉素亲和素-生物素-过氧化酶复合物(SABC)法对肺组织细支气管上皮细胞TGF-β1蛋白表达进行免疫组织化学染色,采用MIAS-2000医学图像分析系统,进行蛋白半定量分析,以最大灰度值反映TGF-β1蛋白表达量。剩余肺组织用4%多聚甲醛溶液固定12 h,用PBS液冲洗,石蜡包埋,制备石蜡组织切片,进行病理学观察。结果两组大鼠动脉氧分压无显著差异(P>0.05),实验组肺组织TGF-β1蛋白含量为(15.66±1.30),对照组蛋白含量为(3.10±0.95),实验组TGF-β1蛋白含量明显高于对照组(P<0.001),实验组肺组织病理切片可见肺组织水肿。结论机械通气可导致肺损伤,转化生长因子-β1可能在其发生、发展过程中起重要作用。Objective To investigate the role of transforming growth factor-β1（TGF-β1）in ventilator induced lung injury（VILI）,and to explore VILI mechanisms.Methods Forty healthy male Wistar rats were randomly divided into the control group（without mechanical ventilation after intubation） and experimental group（after intubation mechanical ventilation：tidal volume 20 ml·kg-1,frequency of 50 times/min,respiratory absorption ratio of 1∶ 3,FiO2 21%,ventilation Time：5 h）,changes in arterial partial pressure of oxygen observed before and after experiment.We took the central part of the lung tissue from left lung,produced lung tissue,and used streptavidin-biotin-peroxidase complex（SABC） method on lung bronchial epithelial cells,immunohistochemical staining of condncted TGF-β1 protein expression,used MIAS-2000 image analysis system for semi-quantitative analysis of proteins,and used the maximum gray value to show the amount of TGF-β1 protein expression.The remaining lung tissue was fixed with 4% paraformaldehyde solution,for 12 h,washed with PBS,embedded in paraffin to prepare paraffin tissue sections for pathological examination.Results There were no significant differences in arterial partial pressure of oxygen（P0.05）.In the experimental group lung tissue TGF-β1 protein content was（15.66±1.30）,while protein content of the control group was（3.10±0.95）.TGF-β1 protein in the experimental group was significantly higher（P0.001）,and lung edema can be seen in lung tissue biopsy.Conclusions Mechanical ventilation can cause lung injury.TGF-β1 may,in its genesis and development,plays an important role.

关 键 词：转化生长因子-Β1 机械通气 肺损伤 机制 

分 类 号：R459.7[医药卫生—急诊医学]