毛细管电泳-激光诱导荧光分析仪组装与性能测试  

Assembly and function test of capillary electrotrophoresis-laser-induced fluorescence

作　　者：余长柱[1] 姜同翠[1] 马云鹏[1] 吴婷妮[1] 李俊[1] 

机构地区：[1]安徽医科大学药学院,安徽合肥230032

出　　处：《安徽医药》2012年第12期1766-1767,共2页Anhui Medical and Pharmaceutical Journal

基　　金：安徽省自然科学基金(No11040606M192);安徽医科大学博士科研基金(NoXJ200805);安徽医科大学校科研基金(No2010XKJ009)

摘　　要：目的组装一台毛细管电泳-激光诱导荧光分析仪。方法以473 nm半导体泵浦固体激光器为激发光源,自制的激光诱导荧光检测器采用共线型光学结构,用VB2为模型化合物进行该分析仪的性能评价。结果 VB2的浓度检出限为2.0×10-9mol·L-1(7.5×10-7 g·L-1),质量检出限为4.0×10-18 mol(1.5×10-15 g),在1.0×10-8～1.0×10-6 mol·L-1浓度范围内呈线性相关。结论自组装毛细管电泳-激光诱导荧光分析仪具有结构简单,操作方便,灵敏度高等优点。Objective To assemble a simple capillary electrotrophoresis-laser-induced fluorescence（CE-LIF） analyzer.Methods A 473-nm MBL-10 diode pumped solid-state laser was employed as an excitation source.The analytical system was based on the collinear optic configuration arrangement.VB2 was used as model compound to evaluate the analytical characteristics.Results The concentration detection limit（CLOD） and mass detection limit（MLOD） of VB2 was 2.0×10-9 mol·L-1（7.5×10-7 g·L-1） and 4.0×10-18 mol（1.5×10-15 g）,respectively.The linear calibration range was from 1.0×10-8 to 1.0×10-6 mol·L-1.Conlusion The home-made CE-LIF analyzer is simple to assemble and convenient to carry out,which has a very high detection sensitivity.

关 键 词：激光诱导荧光 毛细管电泳 VB2 

分 类 号：R318.6[医药卫生—生物医学工程]