不同干燥方法对黄芩叶中黄酮类成分的影响研究  

The Effect of Different Drying Methods on Contents of Flavonoids of Scutellaria baicalensis Georgi Leaves

作　　者：何春年[1,2] 肖苏萍[3] 田壮[3] 曾燕[3] 赵润怀[3] 彭勇[1,2] 肖培根[1,2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]国家教育部中草药物质基础与资源利用重点实验室,北京100193 [3]中国药材公司,北京100195

出　　处：《中国现代中药》2012年第12期31-35,共5页Modern Chinese Medicine

基　　金：中药材产地加工技术平台子课题-河北道地药材黄芩产地加工技术示范研究[(工信部消费(2011)340)];中医药行业科研专项--黄芩道地药材特色栽培及加工技术整理;规范及应用(201107009)

摘　　要：目的:比较不同干燥方法对黄芩叶有效成分含量的影响,优选黄芩叶的最佳干燥方式。方法:以比色法测定总黄酮含量,HPLC法测定6种主要黄酮成分:野黄芩苷、黄芩苷、汉黄芩苷、芹菜素、黄芩素和汉黄芩素的含量,比较自然晾晒、室内通风阴干、烘箱鼓风干燥、真空干燥和微波干燥等5种干燥方法处理黄芩叶的优劣。结果:采用微波干燥的方式黄芩叶总黄酮含量最高,含量23.00%;野黄芩苷在黄芩叶中含量较高,其中以微波干燥最高(3.62%),真空干燥最低(2.77%);微波连续加热会导致总黄酮和野黄芩苷含量下降。结论:从成本、有效成分含量和实际操作综合分析,黄芩叶应采用自然晒干或自然晾干的方法。Objective： To compare the effect of different drying methods on contents of active ingredients from leaves of Scutellaria baicalensis, and find out optimum drying method for S. baicalensis. Methods： The leaves ofS. baicalensis was processed by five drying methods including natural drying in the sun, natural drying in the shade, oven drying under 60 ℃, vacuum drying and microwave drying. Total flavonoids and 6 flavonoids constituents including scutellarin, baicalin, wogonoside, apigenin, baicalein and wogonin were determined by using UV and HPLC method, respectively. Results： The content of total flavonoids was the highest （ average content was 23.00% ） by microwave drying. The content of Scutellarin was higher in leaves of S. baicalensis, which was the highest by microwave drying （3.62%） and was the lowest by vacuum drying （2. 77% ）, respectively. However, the contents of total flavonoids and scutellarin were decreased by microwave in long time heating. Conclusion： On the basis of comprehensively analyzing, the content of flavonoids and actual operation, natural dry in the sun or natural air-dry method should be used in drying the leaves of S. baicalensis.

关 键 词：黄芩叶 黄芩茶 干燥方法 总黄酮 野黄芩苷 

分 类 号：R283.1[医药卫生—中药学]