重金属及有机物复合污染对大豆生理特性和DNA损伤效应的影响  

Effects of Heavy Metal and Organic Compound Contamination on Physiological Character and DNA Damage of Soybean Seedlings

作　　者：陈璨[1] 常云霞[1] 古红梅[1] 李俐俐[1] 陈龙[1] 

机构地区：[1]周口师范学院生命科学系,河南周口466001

出　　处：《大豆科学》2012年第6期915-919,共5页Soybean Science

基　　金：河南省教育厅自然科学研究计划资助项目(2009B180029;2011B180057);周口师范学院生物化学与分子生物学重点学科建设项目

摘　　要：采用沙培方法研究了模拟复合污水(汞、铜、镉、苯、二甲苯和苯酚)对野生大豆和栽培大豆幼苗的生理特性以及DNA损伤效应的影响。结果表明:经污水处理后,大豆幼苗叶片中叶绿素含量、DNA提取量均下降;DNA增色效应、可溶性蛋白、可溶性糖、脯氨酸、丙二醛含量均上升。其中野生大豆ZKDC的叶绿素含量、DNA提取量下降幅度最小,可溶性蛋白、可溶性糖、脯氨酸含量上升幅度最大,DNA增色效应与丙二醛含量上升幅度最小。因此供试材料中野生大豆ZKDC的耐污能力最强。The effects of sewage（Hg,Cu,Cd,benzene,xylene and phenol）irrigation on physiological character and DNA damage of Glycine soja and Glycine max were evaluated by sand culture tests.The results showed that the contents of chlorophyll and the extracts of DNA of soybean seedlings were significantly decreased than those of control.The hyperchromicity of DNA and the contents of soluble protein,soluble sugar,proline and MDA were significantly increased.Compared with the other tested materials,the Glycine soja＇ZKDC＇had the smallest decline for chlorophyll content and DNA extracts amount,the largest rise for soluble protein,soluble sugar and proline content,and the smallest rise for hyperchromicity of DNA and MDA content.In summation,＇ZKDC＇ has the best torlerance for sewage pollution.

关 键 词：野生大豆 重金属 生理特性 DNA损伤 

分 类 号：S565.1[农业科学—作物学]