施氮对结瘤和非结瘤近等位基因大豆生长和固氮性状的影响  

Effects of Nitrogen Application on Growth and Nitrogen Fixation in Nodulation and Non-nodulation Soybean Isolines

作　　者：石海[1,2] 苗淑杰[1] 刘居东[1] 周克琴[1] 

机构地区：[1]中国科学院东北地理与农业生态研究所黑土区农业生态重点实验室,黑龙江哈尔滨150081 [2]中国科学院研究生院,北京100049

出　　处：《大豆科学》2012年第6期961-965,971,共6页Soybean Science

基　　金：中国科学院青年人才项目(KZCX2-EW-QN306);国家自然科学基金项目(41101219)

摘　　要：以结瘤和非结瘤近等位基因大豆品系为材料,利用砂培方式,分析了不同供氮水平对大豆生长、碳氮代谢、结瘤和固氮的影响。结果表明:供氮水平直接影响大豆生物量,结瘤大豆在N2水平(54 mg尿素·kg-1砂)时生物量达到最大(9.16 g·株-1);非结瘤大豆在N4水平(216 mg尿素·kg-1砂)时生物量达到最大(6.36 g·株-1)。植株体内碳氮含量与生物量的变化趋势相同。此外,结瘤品系N2水平大豆根瘤数最多(205个.株-1),极显著高于除N4以外的其它处理(P<0.01),该水平的共生固氮量最大,为275.92 mg·株-1,且与N3、N4、N5处理间差异极显著(P<0.01)。因此,结瘤和非结瘤大豆对外源氮的需求存在显著差异,该试验条件下的氮阻遏阈值为N2水平,即54 mg尿素·kg-1砂。To illustrate the influence of nitrogen fertilizer application on the growth and nitrogen fixation in soybean,the study on the effect of the different N application levels on growth,carbon and nitrogen metabolism,nodulation and nitrogen fixation in soybean under sand culture condition,was carried out with nodulation and non-nodulation soybean isolines.The results showed that N application levels directly impacted soybean biomass.The biomass of nodulation soybean reached the maximum value of 9.16 g·plant-1 at N2 level（54 mg urea·kg-1 sand）,while the non-nodulation soybean reached the maximum of 6.36 g·plant-1 at N4 level（216 mg urea·kg-1 sand）.There was similar trend of carbon and nitrogen metabolism affected by N level.In addition,nodule number reached the largest number（205 per plant）at N2 level,and there were significant differences between N2 and other treatment,exception of N4 level（P0.01）.The amount of symbiotic nitrogen fixation reached the maximum of 275.92 mg·plant-1 at N2 level,and there were significant differences between N2 and N3（N4 or N5）（P0.01）.These results showed that there was obvious difference between nodulation and non-nodulation soybean isoline to the demand of exogenous nitrogen.To take full advantage of symbiotic nitrogen fixation of soybean,nitrogen repression threshold was N2 level（54 mg·kg-1 sand）under this experiment conditions.

关 键 词：C、N代谢 结瘤 固氮 近等位基因大豆 阈值 

分 类 号：S565.1[农业科学—作物学]