微柱凝胶法检测孕妇血清IgG抗A（B）抗体的参考效价推算  

Reference titer of anti-A （B） IgG antibody in serum of pregnant women with microcolumn gel assay

作　　者：蒋璐茜[1] 陈秉宇[1] 李育[1] 谢一唯[1] 冯秋君[1] 

机构地区：[1]浙江省人民医院输血科,杭州310014

出　　处：《浙江医学》2012年第22期1812-1814,共3页Zhejiang Medical Journal

摘　　要：目的分析比较微柱凝胶法与传统抗人球蛋白法检测孕妇IgG抗A（B）抗体效价的相关性和线性数量关系，推算相应的微柱凝胶法参考值。方法分别采用微柱凝胶法和传统抗人球蛋白法检测199例门诊和住院0型孕妇IgG抗A（B）效价，并结合其中58例新生儿ABO溶血病检测结果进行统计分析。结果微柱凝胶法检测阳性率达79．9％，传统抗人球蛋白法阳性率达64.8％，两者有统计学差异（P〈0．01）。两种方法的结果呈高度正相关（r=0．869，P〈0．01）。推算微柱凝胶法相应的参考理论效价为114，与新生儿溶血病临床资料分析较相符。结论本研究推算的理论参考效价有助于今后微柱凝胶法医学决定值的建立。Objective To calculate the reference value of anti-A （B） IgG antibody in saerum of pregnant women with mi- crocolumn gel assay. Methods The methods of microcolumn gel assay and traditional Coomb＇s test were used to measure the titer of anti-A （B） IgG in 199 pregnant women of blood type O. Combined with the test resutts of 58 cases of newborn ABO hemolytic diseases the results were analyzed by statistics software SPSS 18.0. Results The positive rate of microcolumn gel as- say was 79.9%, while that of traditional Coomb＇s test was 64.8%（P〈0.01）; Pearson Chi-square test showed a highly positive cor- relation between the two methods （r=0.869,P〈0.01）. The calculated reference value of microcolumn gel assay was 114, also was consistent with clinical data analysis, Conclusion The calculating reference titer of anti-A （B） IgG will be of value for application of microcolumn gel assay in future research and clinical practice.

关 键 词：微柱凝胶法 IGG抗体 参考效价 

分 类 号：R722.18[医药卫生—儿科]