核转录因子kappaB在急性心肌梗死后心室重塑中作用研究  

Study about the effective of nuclear factor kappa-B in the Left ventricular remodeling after myocardial infarction.

作　　者：樊冬梅[1] 薛莉[2] 

机构地区：[1]陕西省宝鸡市中心医院,宝鸡721008 [2]宁夏医科大学附属医院

出　　处：《陕西医学杂志》2012年第12期1582-1585,共4页Shaanxi Medical Journal

摘　　要：目的:探讨外周血单个核细胞(PBMCs)核因子-kappaB(NF-κB)活化程度对急性心肌梗死(AMI)后左心室重塑(LVRM)作用及其与血浆肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)分泌的相关性。方法:选取首次AMI患者58例,进行超声心动图检查,按simpson法计算出的左心室舒张末期容积,并计算出左心室舒张末期容积指数增加率(ΔLVEDVI),以△LVEDVI>20%将患者分为心室重塑组33例和无心室重塑组25例;另取20例体检正常者为对照组。三组PB-MCs的NF-κB活性用细胞免疫化学染色检测,血浆细胞因子TNF-α,IL-1β含量由放射免疫法测定。结果:①AMI重塑组血浆外周血PBMCs的NF-κB核染色阳性百分率、TNF-α、IL-1β分泌表达均显著增加,高于无重塑组及健康对照组(P<0.01),差异有统计学意义。②左心室重塑组患者外周血PBMCs的NF-κB核染色阳性百分率与TNF-α、IL-1β表达均显著正相关(P<0.05)。结论:AMI后心室重塑患者外周血PBMCs的NF-κB活性是明显升高的,可能是通过促进细胞因子(TNF-α、IL-1β)的分泌来参与心室重塑的发病机制。objection ：To investigate the role of NF-kB activation in the development of the Left ventricu- lar remodeling after myocardial infarction, relationship of NF-kB activation on peripheral blood mononuclear cell （PBMCs） and the level of TNF-α,IL -1β. Methods： fifty - eight acute myocardial infarction patient were selected; and carried out echocardiography; and then,calculated to left ventricular end-diastolic volume（refered to mathed of Simpson） ,increase index ratio of the left ventricular end-diastolic volume. According to left ventricular end-diastolic volume index increasing exceed to 20 percent ,the acute myocardial infarction patient were divide in to two groups, ventricular remodeling group and no ventricular remodeling group; twenty normal human defined as control group; the activity of NF-kB was measured by immunocytochemical staining, plasma cell eytokine （TNF-αand IL-1β）were measured with radioimmunity assay. Results： ①Compared with the normal control group and no ventrieular remod- eling group, NF-KB activation ,TNF-α and IL -19 levels in the ventricular remodeling after the acute myocardial in- farction was bonded to be increased （P〈0.01）. Discrepancy has statistical significance. ② NF-KB activation were positively correlated with the level TNF-α and IL-1β（P〈0.05） . Conclution： Increased NF-KB activation in ventric- ular remodeling after acute myocardial infarction may be upregulate cytokine . as it was, NF-KB take part in patho- physiology of the heart failure and ventricles remodeling after acute myocardial infarction .

关 键 词：心肌梗塞 心室复建 核因子ΚB 

分 类 号：R542.2[医药卫生—心血管疾病]