ACL-TOP全自动血凝仪纤维蛋白原测定的方法学评价  

The methodology evaluation of fibrinogen determination on ACL-TOP automatic coagulation analyzer

作　　者：陈晓蓓[1] 

机构地区：[1]宁波市第一医院检验科,浙江宁波315010

出　　处：《检验医学》2012年第12期1021-1023,共3页Laboratory Medicine

摘　　要：目的通过对ACL-TOP全自动血凝仪测定纤维蛋白原(Fib)的2种方法学进行比较,评价两者检测结果的相关性和准确性,为临床实验室的方法学选择提供依据。方法采用ACL-TOP全自动血凝仪的VonClauss法和PT-der法分别测定凝血酶原时间(PT)正常组和口服抗凝剂组Fib含量;对1份高Fib标本用因子稀释液进行稀释,具体稀释比例依次为1∶8、2∶7、3∶6、4∶5、5∶4、6∶3、7∶2、8∶1、原倍,然后以稀释比例倍数为横坐标,以2种方法测得的Fib含量为纵坐标分别进行简单线性回归。结果无论PT正常组还是口服抗凝剂组Fib测定的2种方法差异均有统计学意义(P=0.000),Fib PT-der法所得结果明显高于Von Clauss法;2种方法的线性关系显示,Von Clauss法近乎为完美且基本过原点的直线,要优于PT-der法。结论血浆Fib PT-der法测定的结果高于Von Clauss法,且差异有统计学意义;Von Clauss法能真实反映人体内具有凝血功能的Fib水平,建议采用美国临床实验室标准化委员会(NCCLS)推荐的Von Clauss法作为临床实验室测定的常规方法。Objective To compare 2 methods with ACL-TOP automatic coagulation analyzer for the determination of fibrinogen （Fib） , to evaluate the correlation and accuracy of test results ,and to provide the reference for the clinical laboratory methods. Methods Von Clauss method and PT-der method with ACL-TOP automatic coagulation analyzer were used to detect the Fib levels of normal PT group and the oral anticoagulant group. A high-concentration Fib sample was diluted with factor diluent. The specific dilution ratios were in turn 1： 8,2： 7,3： 6,4： 5,5： 4,6： 3,7： 2,8：1 and former times. The dilution ratio was as the abscissa, and the Fib level was as vertical axis measured by the 2 methods, respectively. A simple linear regression was performed. Results The Fib levels of the normal PT group or oral anticoagulant group by the 2 methods were different significantly （ P = 0. 000）. The results of Fib by PT-der method were significantly higher than those by Von Clauss method. The linearity of the 2 methods showed that the linearity of Von Clauss method was almost perfect, which was superior to that of PT-der method. Conclusions The results of PTder method for Fib determination are higher than those of Von Clauss method with statistical significance. Von Clauss method can reflect the Fib levels. The Von Clauss method recommended by National Committee for Clinical Laboratory Standards （NCCLS） should be as the conventional method of clinical measurement.

关 键 词：纤维蛋白原 Von CLAUSS法 PT-DER法 线性 

分 类 号：R446.11[医药卫生—诊断学]