疤痕止痒软化膏对烧伤创面致痒介质影响的动物实验研究  

Experimental study on effect of cicatrix antipruritic ointment on burn wounds itch mediators

作　　者：易先锋[1] 卢根香[2] 宋春红[1] 钟伟湘[1] 童钟[1] 

机构地区：[1]广东省工伤康复中心,广东广州510440 [2]广东科学技术职业学院,广东广州510432

出　　处：《中国热带医学》2012年第11期1310-1312,1319,共4页China Tropical Medicine

基　　金：广东省科技立项课题(No.2010B031600251)

摘　　要：目的研究疤痕止痒软化中药膏剂对家兔烧伤创面致痒介质的影响。方法通过Cajal吡啶银染法,免疫组织化学技术,计算机辅助图像分析技术检测使用疤痕止痒软化膏后家兔烧伤创面及创面愈合后不同时间组织神经末梢数量,(substance P,SP)P物质,肥大细胞数目,组胺及5-羟色胺的多少。结果家兔使用疤痕止痒膏在烧伤创面及烧伤愈合后的创面不同时间点上部分毁损皮肤的神经末梢整体结构及数量实验组与对照组比较差异性不大。用药后家兔深Ⅱ°烧伤创面及烧伤愈合后创面组织不同时间点中组织SP含量与对照组比较差异性不大(P﹥0.01)。用药后实验组脱颗粒的肥大细胞数目明显少于对照组(P<0.01)。实验组的组织胺及5-色胺量与对照组比较有明显差异(P<0.01)。结论疤痕止痒软化膏对家兔烧伤创面致痒介质有一定的影响,从烧伤创面早期就开始用药物进行烧伤瘙痒的干预是一条可以考虑的方法。Objective To observe the effects of cicatrix antipruritic ointment on burn wounds itch mediators of the rabbit.Methods The contents of nerve endings,substance P,mast cell,histamine and serotonin of the rabbits burn wounds treated by cicatrix antipruritic ointment in different time were determined by using Cajal Pyridine silver staining,immunohistochemical techniques and the computer image processing technique,we finished the study.Detecting the.Results The differences of nerve endings and SP content between two groups at the different time periods of the burn wounds were insignificant（P﹥0.01）.The differences between two groups were significant（P 0.01）.Conclusion There is a certain impact of Antipruritic ointment for cicatrix preparation on the rabbit burn wounds itch mediators.Early intervention of burn wounds early period for burn itching is a method can be considered.

关 键 词：动物实验 烧伤创面 瘙痒 疤痕止痒软化膏 干预 

分 类 号：R644[医药卫生—外科学]