戊脉安等钙拮抗剂对平阳霉素细胞毒性的生化调节作用  

BIOCHEMICAL MODULATION ON PINGYANGMYCIN CYTOTOXICITY BY VERAPAMIL AND OTHER CALCIUM ANTAGONISTS

作　　者：赵小平[1] 刘东平[1] 吴德政[1] 

机构地区：[1]军事医学科学院附属医院临床药理研究室,北京100850

出　　处：《癌症》1991年第3期184-186,共3页Chinese Journal of Cancer

摘　　要：本文在体外培养的L_(1210)细胞中观察了平阳霉素单用或与钙拮抗剂合用时的细胞毒作用,结果表明:戊脉安本身在无细胞毒性浓度下与平阳霉素联合应用时,可显著加强平阳霉素(75μg/ml)的细胞毒作用。而且这种增效作用随戊脉安浓度的增加而加强,细胞存活率可由平阳霉素单药时的25％,分别降低为0.11％-9.9％。同样,当戊脉安浓度固定为20μg/ml时,增效作用随着平阳霉素浓度的增加而加强。此外,还发现钙调蛋白阻滞剂利多卡因、氯丙嗪及普鲁卡因,对平阳霉素也具有类似的增效作用,其增效幅度也随着上述各种钙阻滞剂的浓度增加而加强。Soft agar colony-forming assay was used to determine the cytotoxi-city of Pingyangmycin (PYM) alone or in combination with calcium antagonist Verapamil (Vera), Lidocaine, chlorpromazine, or procaine. In vitro experimental results showed that Vera under non-toxic concentration could potentiate the PYM cytotoxicity to L1210 cells significantly. The concentration of Vera was linearly correlated with the cell survival of the Vera-PYM treated group (r=-0.8763). The cell survival fraction was 25% for PYM alone and 0.11% -9.9% for PYM in combination with various concentrations of Vera. If the Vera concentration was fixed at 20μg/ml the cytotoxicity of PYM was increased with the increased concentrations of PYM. The experimental data also indicated that lidocaine, chlorpromazine and procaine could also potentiate the PYM cytotoxicity. The potentiating effect was also linearly correlated with the concentration of above mentioned calcium antagonists.

关 键 词：戊脉安 钙拮抗剂 平阳霉素 药理学 

分 类 号：R969.2[医药卫生—药理学]