Preparation and Antibacterial Activity of Copolymer of Methacryloxyethyldimethyl Dodecyl Ammonium Bromide/Acrylamide  

作　　者：LU Gui-qian ZUO Hua-jiang DONG Wei-min WU Ding-cai FU Ruo-wen 

机构地区：[1]Key Laboratory for Polymeric Composite and Functiond Materials, Ministry of Education [2]Design and Synthesis of New Polymer Materials and Application Laboratory, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, P. R. China

出　　处：《Chemical Research in Chinese Universities》2012年第6期1095-1100,共6页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.50673101);the Project of the Department of Education of Guangdong Province,China(No.cgzhzd0901)

摘　　要：Copolymers PMDAB-co-AA with excellent antibacterial activity were designed and prepared（MDAB/AA=methacryloxyethyldimethyl dodecyl ammonium bromide with acrylamide）.The chemical structures of the copolymers were characterized by Fourier transform infrared（FTIR）,1 H NMR,13 C NMR and chemical titration.The antimicrobial activities and kinetics of the copolymer against E.coli and S.aureus were examined by viable cell counting method.The compositions of the copolymers could be easily controlled by the feed molar ratio of MDAB to AA.Furthermore,the highest antibacterial activity was achieved when the molar fraction of MDAB was in a range of 5%―20%,and the killing rate reached 100% under the test conditions.In addition,the antibacterial activity of PMDAB-co-AA was maintained and stable without any loss after 15 times of repeated usage.It was proved that the PMDAB-co-AA samples targeted at cell membrane,and caused serious damage to cell integrity and inner membrane permeation.The surrounding conditions,such as pH and inorgainc salts concentrations（CaCl2 or NaCl）,also affected their antibacterial activities.Copolymers PMDAB-co-AA with excellent antibacterial activity were designed and prepared（MDAB/AA=methacryloxyethyldimethyl dodecyl ammonium bromide with acrylamide）.The chemical structures of the copolymers were characterized by Fourier transform infrared（FTIR）,1 H NMR,13 C NMR and chemical titration.The antimicrobial activities and kinetics of the copolymer against E.coli and S.aureus were examined by viable cell counting method.The compositions of the copolymers could be easily controlled by the feed molar ratio of MDAB to AA.Furthermore,the highest antibacterial activity was achieved when the molar fraction of MDAB was in a range of 5%―20%,and the killing rate reached 100% under the test conditions.In addition,the antibacterial activity of PMDAB-co-AA was maintained and stable without any loss after 15 times of repeated usage.It was proved that the PMDAB-co-AA samples targeted at cell membrane,and caused serious damage to cell integrity and inner membrane permeation.The surrounding conditions,such as pH and inorgainc salts concentrations（CaCl2 or NaCl）,also affected their antibacterial activities.

关 键 词：Dimethylaminoethyl methacrylate ACRYLAMIDE Quaternary ammonium salt Antibacterial activity 

分 类 号：O647.1[理学—物理化学] TQ314.253[理学—化学]