Effect of Chinese Drugs for Activating Blood Circulation and Detoxifying on Indices of Thrombosis,Inflammatory Reaction,and Tissue Damage in A Rabbit Model of Toxin-Heat and Blood Stasis Syndrome  

作　　者：薛梅 殷惠军 吴彩凤 马晓娟 郭春雨 黄烨 史大卓 陈可冀 

机构地区：[1]Department of Cardiovascular Diseases,Xiyuan Hospital,China Academy of Chinese Medical Sciences

出　　处：《Chinese Journal of Integrative Medicine》2013年第1期42-47,共6页中国结合医学杂志（英文版）

基　　金：Supported by National Basic Research Program of China(No. 2006CB504803 and No.81030063)

摘　　要：Objective： To observe the regulatory effect of Chinese drugs for activating blood circulation （ABC） and for activating blood circulation and detoxifying （ABCD） on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome. Methods： Fifty-four rabbits were randomized into the normal control group, model group, simvastatin group （simvastatin, 0.93 mg/kg per day）, ABC group [Xiongshao Capsule （~ff_）, 0.07 g/kg per day], and ABCD group [Xiongshao Capsule, 0.07 g/kg per day, and Huanglian Capsule （-~L^t）, 0.14 g/kg per day]. All except the normal control group received a single injection of bovine serum albumin and were fed with high-fat diets for 6 weeks. At the end of week 4 of giving high- fat diets, a dose of endoxitin was given by ear vein injection, and a randomized 2-week treatment was initiated. At the end of treatment, blood lipids, circulating endothelial cells, and the pathological changes of the aortic arch were assessed. The serum levels of matrix metalloproteinases （MMP-9）, tissue inhibitors to metalloproteinase （TIMP-1）, granule membrane protein-140 （GMP-140）, plasminogen activator inhibitor-1 （PAl-l）, high-sensitivity C-reactive protein （hs-CRP）, interleukin-6 （IL-6）, and tumor necrosis factor- ~ （TNF- oL ） were determined. Results： Compared with the model group, ABCD group showed decreased serum triglycaride （TG） level, improvement in the pathological change in the aortic arch, and reduction in the number of circulating endothelial cells （4.00_＋ 1.41 per 0.9 p~ L for ABCD group vs 7.83_＋ 1.72 per 0.9 μL for the model group）. In addition, the levels of serum GMP-140, PAl-l, and IL-6 in ABCD group were also significantly reduced [0.79＋0.20 ng/mL, 5.23± 1.39 ng/mL, 40.64 ± 10.11 pg/mL for ABCD group vs 1.08 ± 0.31 ng/mL, 7.28± 2.01 ng/mL, 54.44 ± 13.56 pg/mL for the model group, respectively, P〈0.05]. A trend showing improvement in the indices of thrombosis, inflammatObjective： To observe the regulatory effect of Chinese drugs for activating blood circulation （ABC） and for activating blood circulation and detoxifying （ABCD） on indices of thrombosis, inflammatory reaction, and tissue damage in a rabbit model of toxin-heat and blood stasis syndrome. Methods： Fifty-four rabbits were randomized into the normal control group, model group, simvastatin group （simvastatin, 0.93 mg/kg per day）, ABC group [Xiongshao Capsule （~ff_）, 0.07 g/kg per day], and ABCD group [Xiongshao Capsule, 0.07 g/kg per day, and Huanglian Capsule （-~L^t）, 0.14 g/kg per day]. All except the normal control group received a single injection of bovine serum albumin and were fed with high-fat diets for 6 weeks. At the end of week 4 of giving high- fat diets, a dose of endoxitin was given by ear vein injection, and a randomized 2-week treatment was initiated. At the end of treatment, blood lipids, circulating endothelial cells, and the pathological changes of the aortic arch were assessed. The serum levels of matrix metalloproteinases （MMP-9）, tissue inhibitors to metalloproteinase （TIMP-1）, granule membrane protein-140 （GMP-140）, plasminogen activator inhibitor-1 （PAl-l）, high-sensitivity C-reactive protein （hs-CRP）, interleukin-6 （IL-6）, and tumor necrosis factor- ~ （TNF- oL ） were determined. Results： Compared with the model group, ABCD group showed decreased serum triglycaride （TG） level, improvement in the pathological change in the aortic arch, and reduction in the number of circulating endothelial cells （4.00_＋ 1.41 per 0.9 p~ L for ABCD group vs 7.83_＋ 1.72 per 0.9 μL for the model group）. In addition, the levels of serum GMP-140, PAl-l, and IL-6 in ABCD group were also significantly reduced [0.79＋0.20 ng/mL, 5.23± 1.39 ng/mL, 40.64 ± 10.11 pg/mL for ABCD group vs 1.08 ± 0.31 ng/mL, 7.28± 2.01 ng/mL, 54.44 ± 13.56 pg/mL for the model group, respectively, P〈0.05]. A trend showing improvement in the indices of thrombosis, inflammat

关 键 词：activating blood circulation activating blood circulation and detoxifying THROMBOSIS tissuedamage inflammatory reaction 

分 类 号：R285[医药卫生—中药学]