蛇足石杉内生真菌抗乙酰胆碱酯酶活性筛选及分类鉴定  

Screening of endophytic fungi from Huperzia serrata for acetylcholinesterase inhibitory activity and its taxonomic identification

作　　者：王莉莉[1,2] 吕会芳[1,2] 张丽[1,2] 化海霞[1,2] 王杰华[1,2] 胡之璧[1,2] 黎万奎[1,2] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室,上海201213 [2]上海中医药大学中药研究所中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海201213

出　　处：《中国中药杂志》2012年第24期3701-3705,共5页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30873386;81130070);上海高校优青基金项目(szy10036)

摘　　要：目的:从蛇足石杉Huperzia serrata(Thunb.ex Murray)Trevis.内生真菌中筛选具有抗乙酰胆碱酯酶活性的菌株。方法:利用乙酰胆碱酯酶水解α-萘乙酯的产物和固蓝B盐发生显色反应的原理,用薄层色谱-生物自显影法对59株蛇足石杉内生真菌发酵产物进行抗乙酰胆碱酯酶活性筛选,并通过18s rDNA和5.8s rDNA序列分析结合形态学特征对目标菌株进行分类鉴定。结果:蛇足石杉内生真菌LQ2F01在抗乙酰胆碱脂酶活性筛选中呈现阳性颜色反应,18s rDNA和5.8s rDNA序列分析并结合形态学分类表明该菌株属枝顶孢霉属Acremonium。结论:蛇足石杉内生真菌LQ2F01表现出和宿主植物相同的抗乙酰胆碱脂酶活性,在天然药物开发以及内生真菌与宿主植物关系的研究中具有重要意义。Objective： To screen out fungus strains with acetylcholinesterase inhibitory activity from Huperzia serrata. Method ： Endophytic fungi fermentation products from 59 H. serrata strains were stained with acetylcholinesterase hydrolyzed a-naphthalenecetic ethyl ester and fast blue B salt, and screened for acetylcholinesterase inhibitory activity with thin-layer chromatography-bioautography. Target strains were classified and identified through the sequence analysis on 18s rDNA and 5.8s rDNA combined with morphological characteristics. Result： Fungus strain LQ2F01 from H. serrata showed positive color reaction in the screening for acetylcho- linesterase inhibitory activity. The sequence analysis on 18s rDNA and 5.8s rDNA combined with morphological characteristics showed the strain LQ2F01 belonged to Acremonium. Conclusion ： Endophytic Fungi LQ2F01 from H. serrata shows identical acetylcholinesterase inhibitory activity with the host plant, which is of great significance to the development of natural medicines and the studies on the relationship between the endophytic gungi and the host plant.

关 键 词：蛇足石杉 内生真菌 乙酰胆碱酯酶抑制剂 RDNA 枝顶孢霉属 

分 类 号：R284[医药卫生—中药学]