太子参不定根组织培养的研究  

Study on tissue cultivation of adventitious roots of Pseudoxtellariae heterophylla

作　　者：梁玉勇[1] 尹双双[2] 左北梅[3] 高文远[2,3] 

机构地区：[1]铜仁职业技术学院,贵州铜仁554300 [2]天津大学药物科学与技术学院,天津300072 [3]天津科技大学中药生物工程研究所,天津300457

出　　处：《中国中药杂志》2012年第24期3803-3807,共5页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划专项(2006BAI06A16-02)

摘　　要：目的:对太子参不定根的培养条件进行系统的优化。方法:利用组织培养技术结合紫外分光光度法,考察了接种量、蔗糖浓度、无机盐浓度、培养天数、逐级扩大培养以及不同角度鼓泡式反应器对太子参不定根生长的影响,并对不定根中皂苷、多糖和氨基酸等成分进行含量测定。结果:每1 L培养基接种的不定根鲜重为6 g时,太子参不定根的干重增殖倍数达到最大值;随着蔗糖浓度的升高,太子参不定根干重增殖倍数呈先升高后降低的趋势;培养基中的无机盐浓度对不定根的生长有较大影响,3/4 MS最有利于不定根的生长;太子参生长呈"S"型曲线,在第28天生物量达到最大。结论:接种量、蔗糖浓度、无机盐浓度、逐级扩大培养以及反应器角度都会显著影响太子参不定根的生长,不定根中的皂苷和氨基酸含量高于栽培的太子参,而多糖含量低于栽培的太子参。Objective： To systematically optimize the cultivation conditions of adventitious roots of Pseudoxtellariae heterophylla. Method ： Tissue cultivation technology and ultraviolet spectrophotometry were adopted to observe the effect of inoculum volume, sucrose concentration, inorganic salt concentration, number of cultivation days, gradual scale-up cultivation and bubble different angles of bioreactor on the growth of adventitious roots of P. heterophylla, and determine the content of constituents such as saponin, polysaccharide and amino acid. Result： The propagation multiple of adventitious roots reached the maximum when the inoculum was 6 g in a 1 L culture shake flask. With the increase in sucrose concentration, the dry weight propagation multiples of adventitious roots followed an up and down trend. The inorganic salt concentration in a cultivation dish had a greater effect on the growth of adventitious roots, partic- ularly 3/4 MS was the most favorable for the growth of adventitious roots. The growth curve of P. heterophylla was ＂S＂, with the bio- mass reaching the maximum at the 28th day. Conclusion： The inoculum volume, sucrose concentration, inorganic salt concentration, gradual scale-up cultivation and angles of bubble bioreactor had a significant effect on the growth of adventitious roots of P. heterophylla. The contents of saponin and amino acid in adventitious roots were higher than that in cultivated P. heterophylla, whereas the poly-saccharide content were lower than that in cultivated P. heterophylla.

关 键 词：太子参 不定根 接种量 蔗糖浓度 盐浓度 生长曲线 逐级扩大培养 成分含量 

分 类 号：S567.53[农业科学—中草药栽培] R284[农业科学—作物学]