山东地区汉族人群杀伤细胞免疫球蛋白样受体和白细胞抗原C基因与梅毒的关系  

Association between killer cell immunoglobulin-like receptor and human leukocyte antigen-C genes and syphilis in the Han population in Shandong area

作　　者：乔文本 刘丽[1] 庄云龙[1] 刘虹[1] 周娟[1] 竺青[1] 张毅[1] 刘艳[1] 聂向民[1] 宋永红[1] 朱传福[1] 王大见[2] 

机构地区：[1]山东省血液中心,济南250014 [2]济南市皮肤病防治院

出　　处：《国际免疫学杂志》2013年第1期64-69,共6页International Journal of Immunology

摘　　要：目的分析山东汉族梅毒（Syphilis）患者杀伤细胞免疫球蛋白样受体（KIR）基因和人类白细胞抗原（HLA）C基因多态性，探讨其与梅毒发生之间的关联性。方法采用序列特异性引物聚合酶链反应（PCR—SSP）法，对山东汉族231例梅毒患者和247例健康个体KIR和HLA—C基因进行检测和分析。结果在检测的全部个体中，框架基因KIR2DIA、KIR3DL2、KIR3DL3和KIR3DPI的表型频率均为100％。抑制型K／R基因的表型频率在梅毒病例组和对照组中相比差别无统计学意义。梅毒病例组激活型KIR2DS3和KIR3DS1基因的表型频率均显著高于对照组（P=0．030，P=0．038），而KIR2DS5基因的表型频率显著低于对照组（P=0．015，OR=0．575）。梅毒病例组纯合子（homozygote）HLA—C1C1基因型的表型频率显著低于对照组（P=0．030，OR=0．667）。梅毒病例组HLA—C1C1-KIR2DL3基因型的表型频率显著低于对照组（P=0．018，OR=0．647）。结论KIR2DS3、KIR3DS1基因可能是梅毒的易感基因、KIR2DS5基因，HLA—C1C1和HM—G1C1-KIR2DL3基因型可能是梅毒的抗性基因（型）。Objective To explore whether killer cell immunoglobulin-like receptor （KIR）and human leukocyte antigen （HLA） C gene polymorphisms are associated with syphilis in a Han population in Shandong area . Methods Polymerase chain reaction with sequence-specific primers （PCR-SSP） was used to genotype KIR and HLA-C genes in 231 syphilis patients and 247 healthy controls. Results Framework genes K/R2DL4, KIR3DL2, KIR3DL3 and KIR3DP1 were present in all individuals. There were no significant different distribu- tions of inhibitory K1R genes in the two groups. The frequencies of KIR2DS3 and KIR3DS1 were higher in syph- ilis patients than those in healthy controls （P = 0. 030 and P = 0. 038, respectively） , and the frequency of KIR2DS5 was higher in healthy controls than that in syphilis patients （P =0. 015 ; OR =0.575）. The homozy- gote for HLA-CI allele （ HLA-C1 C1 ） was more common in controls compared to syphilis patients （ P = 0. 030 ; OR --0.667）. The frequency of individuals with HLA-C1C1 and K/R2DL3 genotype was higher in control group relative to syphilis patient group （P = 0. 018; OR = 0. 647 ）. Conclusions These data suggested that KIR2DS3 and KIR3DS1 were associated with susceptibility to syphilis, while KIR2DSS, HLA-C1 C1 and HLA- C1C1-K/R2DL3 were associated with resistance to syphilis in the Han population in Shandong area. However, investigation using functional studies are required.

关 键 词：人类白细胞抗原C基因 杀伤细胞免疫球蛋白样受体 序列特异性引物聚合酶链反应 梅毒 

分 类 号：R7[医药卫生—临床医学]