树舌多糖GF抑制肝癌细胞SMMC7721增殖及对CDK2基因表达的影响  

Effects of GAPSGF on Inhibiting Hepatoma Cell Line SMMC7721 Proliferation and CDK2 Expression

作　　者：刘佳维[1,2] 宋高臣[2] 于水澜[1] 于英君[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]牡丹江医学院,黑龙江牡丹江157011

出　　处：《中医药学报》2012年第6期36-39,共4页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省青年科学技术专项基金项目(No.QC06C090);黑龙江省中医药管理局项目(No.ZHY06-Z02);黑龙江省青年科学基金(No.2010001)

摘　　要：目的:探明树舌多糖GF(Ganoderma appanatum polysaccharides GF,GAPSGF)对肝癌细胞SMMC7721增殖及CDK2表达的影响。方法:不同浓度树舌多糖GF处理肝癌细胞SMMC7721,采用MTT法检测树舌多糖GF对肝癌细胞SMMC7721增殖抑制作用;实时定量聚合酶链反应(Real-TimePCR)检测CDK2 mRNA表达;蛋白印迹法(Western blot)检测CDK2蛋白表达情况。结果:树舌多糖GF对肝癌细胞SMMC7721具有明显的生长抑制作用。其中树舌多糖GF2.5μg/mL组与树舌多糖GF10μg/mL组作用显著,且CDK2的mRNA和蛋白表达下降。结论:树舌多糖GF可能通过降低CDK2表达,抑制肝癌细胞SMMC7721增殖。Objective： To study the effects of Ganoderma appanatum polysaccharides GF（GAPSGF）on proliferation and CDK2 expression in hepatoma cell line SMMC7721.Methods： MTT assay was used to examine the growth and proliferation of SMMC7721 cells after treatment with different concentrations of GAPSGF.Real-time PCR and Western blot were performed to measure the expression of CDK2 at mRNA and protein level.Results： GAPSGF could effectively inhibit proliferation of hepatoma cell line SMMC7721,and GAPSGF at concentration of 2.5μg·mL-1 and 10μg·mL-1 had a significant effect on inhibiting proliferation.The expression of mRNA and protein of CDK2 effectively reduced.Conclusion： GAPSGF restrains proliferation of hepatoma cell line SMMC7721 by reducing CDK2 expression.

关 键 词：树舌多糖 肝癌细胞 增殖 CDK2 

分 类 号：R285.5[医药卫生—中药学] R735.7[医药卫生—中医学]