高效毛细管电泳法测定石杉碱甲片中石杉碱甲含量  

High Performance Capillary Electrophoretic Determination of Huperzine A in Huperzine A Tablets

作　　者：余宇燕[1] 邹艳辉[1] 滕海英[1] 林舒[1] 张红艳[1] 凌伟坚[1] 

机构地区：[1]福建中医药大学药学院,福州350122

出　　处：《理化检验（化学分册）》2012年第12期1392-1394,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：福建省科技厅重点项目(2011Y0035);福建省科技计划项目(2010Y2004)

摘　　要：提出了毛细管电泳法分离测定石杉碱甲片中石杉碱甲含量的方法。以pH为4.6的乙酸盐溶液为电解质溶液,运行电压13kV,于波长310nm处进行紫外检测。石杉碱甲的质量浓度在5.0～60mg.L-1范围内与峰面积呈线性关系。采用该方法对石杉碱甲片样品中石杉碱甲的浓度进行了测定,所得加标回收率在97.8%～98.7%之间,测定值的相对标准偏差(n=5)在0.53%～0.85%之间。A method for the separation and determination of huperzine A in its tablets by capillary electrophoresis was proposed. An acetate buffer solution of pH 4. 6 was used as background electrolyte, and a moving voltage of 13 kV was applied. UV-detection at the wavelength of 310 nm was adopted. 1.inear relationship between values of peak area and mass concentration of huperzine A was kept in the range of 5.0--60 mg ·L^-. The proposed method was applied to the determination of huperzine A in its tablets, giving values of recovery obtained by standard addition method in the range of 97.8%- 98.7%, and RSD＇s （n=5） in the range of 0.53%-0.85%.

关 键 词：高效毛细管电泳法 石杉碱甲片 石杉碱甲 

分 类 号：O657.8[理学—分析化学]