高效液相色谱法同时测定蓝萼香茶菜中蓝萼甲素和蓝萼乙素的含量  

Simultaneous Determination of Glaucocalyxin A and Glaucocalyxin B in Isodon Japonica by HPLC

作　　者：贺晓丹[1] 张志军[1] 贺志安[2] 张积霞[1] 

机构地区：[1]新乡医学院药学院,新乡453003 [2]新乡医学院医学检验系,新乡453003

出　　处：《理化检验（化学分册）》2012年第12期1395-1397,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：河南省教育厅资助课题(2009B310004);新乡医学院大学生科技创新项目(DXSKYKT2011-058)

摘　　要：提出了高效液相色谱法测定蓝萼香茶菜中蓝萼甲素和蓝萼乙素的含量。样品采用甲醇超声提取后,经0.45μm滤膜过滤,滤液供高效液相色谱仪测定。采用Hypersil ODS C18色谱柱(4.6mm×200mm,5μm)分离,用甲醇-水(55+45)溶液为流动相洗脱,用二极管阵列检测器在波长231nm处测定。蓝萼甲素和蓝萼乙素的质量浓度分别在0.018～0.575g.L-1和0.009～0.298g.L-1范围内与峰面积呈线性关系。方法用于蓝萼香茶菜样品的分析,蓝萼甲素和蓝萼乙素的回收率分别为102%和103%,测定值的相对标准偏差(n=6)分别为0.084%和0.071%。HPI.C was applied to the determination of glaucocalyxin A and glaucocalyxin B in isodon japonica. The sample was extracted ultrasonically with methanol, and after filtering through 0.45μm filtering membrane, the solution was used for HPLC analysis. Hypersil ODS Cl8 chromatographic column （4.6 mm×200 mm, 5 μm） was used for separation and a mixture of methanol and water （55 ＋45） was used as the mobile phase. Detection with diode array detector at the wavelength of 231 nm was adopted. I.inear relationships between values of peak area and mass concentration of glaueocalyxin A and glaucocalyxin B were obtained in the ranges of 0. 018--0. 575 g·L^-1 and 0.009--0. 298 g·L^-1 respectively. Values of recovery and RSD＇s （n=6） found were 102% and 0. 084% respectively for glaucocalyxin A, and 103Y0 and 0. 071%0 respectively for glaucocalyxin B.

关 键 词：高效液相色谱法 蓝萼香茶菜 蓝萼甲素 蓝萼乙素 

分 类 号：O652.63[理学—分析化学]