糖肝康对糖尿病脂肪肝模型大鼠肝脏AdipoR_2 mRNA表达的影响  

Effect of Tanggankang on mRNA Expression of AdipoR_2 in the Liver of Diabetic-fatty Liver Rat Model

作　　者：钱秋海[1] 钱卫斌[2] 郭春芳 蔡欣蕊[4] 李群英[4] 王营营[4] 

机构地区：[1]山东中医药大学附属医院内分泌科,济南250011 [2]日本鸟取大学医学部 [3]禹城市人民医院 [4]山东中医药大学第一临床医学院

出　　处：《中国药师》2012年第11期1533-1535,共3页China Pharmacist

基　　金：山东省中医药科学技术研究项目(编号:2011-038);山东省科学技术厅科学技术发展计划项目(编号:003130130)

摘　　要：目的:研究糖肝康对糖尿病脂肪肝模型大鼠肝脏组织中AdipoR_2 mRNA表达的影响。方法:高糖+高脂+小剂量链脲佐菌素腹腔注射建立糖尿病脂肪肝大鼠模型,并设对照组进行观察。药物干预12周后,采用RT-PCR方法检测糖肝康对肝脏组织中AdipoR_2mRNA表达的影响。结果:模型大鼠的肝脏组织中AdipoR_2 mRNA表达水平较低(0.143±0.046),糖肝康可上调其在肝脏组织中的mRNA表达(小剂量组为0.202±0.052,大剂量组为0.212±0.049),与模型对照组比较,差异有统计学意义(P<0.05),且大剂量组表达高于降糖甲对照组(0.176±0.065)。结论:糖尿病脂肪肝的糖脂代谢紊乱可能与AdipoR_2的低水平表达相关,糖肝康可通过上调AdipoR_2 mRNA表达水平,在糖、脂代谢等方面到积极作用,值得进一步深入研究。Objective：To observe the effect of Tanggankang on mRNA expression of AdipoR,in the liver of diabetic-fatty liver rat model.Method：The rat model was made with STZ and high-fat and high-glucose diet.After 12 weeks of drug intervention,the rats were killed and the livers were removed,and the mRNA expression of AdipoR,in the livers was observed by the method of RT-PCR. Result：The mRNA expression of AdipoR2 in the livers was significantly increased,and Tanggankang could lower the expression of AdipoR_2 in the livers in a dose-dependent manner.Conclusion：The abnormal expression of AdipoR,can be one of the mechanisms of diabetic -fatty liver.Tanggankang has a promising protective effect on the liver through adjusting the expression of AdipoR2,which deserves further research.

关 键 词：糖肝康 糖尿病脂肪肝 RT-PCR 脂联素受体2 

分 类 号：R285.5[医药卫生—中药学]