HPLC法测定人血浆中氯诺昔康浓度  

Determination of Lornoxicam in Human Plasma by HPLC

作　　者：李珂珂[1] 吕青志[1] 赵娟娟[1] 张晓帆[1] 关景欣[1] 

机构地区：[1]滨州医学院药学院,山东烟台264003

出　　处：《中国药师》2012年第11期1565-1567,共3页China Pharmacist

基　　金：烟台市科技发展计划项目(编号:2010326)

摘　　要：目的:建立测定人血浆中氯诺昔康浓度的方法。方法:采用高效液相色谱法,以美洛昔康为内标,血浆样品以直接蛋白沉淀法处理,色谱柱为Agilent C_(18)(250mm×4.6mm,5μm),以甲醇-水(含0.05%三乙胺,用HCl调节pH至6.0)(55:45)为流动相,流速为1.0 ml·min^(-1),柱温为室温,检测波长为378nm,进样量为20μl。结果:氯诺昔康血药浓度在2～500 ng·ml^(-1)浓度范围内线性关系良好(r=0.995 0);高、中、低3种浓度的方法回收率为96.29%～104.00%,提取回收率>92.0%,日内、日间RSD分别为2.57%～6.41%和1.73%～6.62%。结论:本方法灵敏度、准确度及回收率均能达到人体血药浓度测定的要求,也可用于临床人体药动学的研究。Objective：To establish a method for the determination of lornoxicam in human plasma.Method：HPLC was adopted. The plasma samples were extracted by direct protein precipitation using meloxicam as the internal standard.The determination was performed on an Agilent C_（18）（250 mm×4.6 mm,5μm） column with the mobile phase consisting of methanol-water（containing 0.05% triethylamine,pH adjusted to about 6.0 by HCl）（55：45） with the flow rate of 1.0 ml ？ min^（-1） at room temperature.The detection wavelength was set at 378 nm and the injection volume was 20 ｜xl.Result;The linear range of lornoxicam was from 2 to 500 ng ？ ml^（-1） （r = 0.995 0）.The method recovery was 96.29%-104.00%and the extraction recovery was above 92.0%.The intra- and inter-day RSD was 2.57%-6.41%and 1.73%-6.62%,respectively.Conclusion：The sensitivity,accuracy and recovery of the method all meet the standard of plasma concentration monitoring,which can be used in the clinical pharmacokinetic study.

关 键 词：高效液相色谱法 氯诺昔康 血药浓度 

分 类 号：R969.1[医药卫生—药理学]