HPLC法同时测定肝复康丸中五味子醇甲等4种成分的含量  

Simultaneous Determination of Four Components Including Schisandrin in Ganfukang Pills by HPLC

作　　者：林远凤[1] 黄燕萍[2] 

机构地区：[1]北海市人民医院,广西北海536000 [2]北海食品药品检验所

出　　处：《中国药师》2012年第11期1588-1590,共3页China Pharmacist

基　　金：北海市科学研究与技术开发计划项目(编号:北科合200901059)

摘　　要：目的:建立高效液相色谱法同时测定肝复康丸中五味子醇甲、五味子酯甲、五味子甲素和五味子乙素等4种成分的含量测定方法。方法:采用Agilent TC-C_(18)色谱柱(250 mm×4.6 mm,5μm);乙腈-水为流动相,梯度洗脱,流速0.8 ml·min^(-1);检测波长:254 nm。结果:五味子醇甲、五味子酯甲、五味子甲素、五味子乙素与其相邻杂质峰能完全分离,五味子醇甲、五味子酯甲、五味子甲素、五味子乙素的线性范围(相关系数)分别为68.62～686.25μg·ml^(-1)(r=0.999 9),15.54～155.40μg·ml^(-1)(r=0.999 8),6.45～64.50μg·ml^(-1)(r=0.999 9)和15.57～155.70μg·ml^(-1)(r=0.999 9)。平均回收率分别为100.80%,100.46%,101.06%和100.55%,RSD为1.31%,1.72%,2.00%和1.67%。结论:本方法简便、准确、重复性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。Objective：To establish the method for determination of schisaiidrin,sehisantheriii,deoxysehizandriii and schisaiidrin B in Ganfukang pills by HPLC.Method：An Agilent TC-C_（18）（250 mm×4.6 mm,5μm） column was used with the mobile phase of acetonitrile-warter with gradient elution.The How rate was 0.8 ml ？ min^（-1） and the detection wavelength was at 254 nm.Result; Schisandrin,sehisantheriii,deoxysehizandrin and schisaiidrin B eould be completely separated from the impurity peaks.The linear range of those four components was 68.62-686.25μg ？ ml^（-1）（r =0.999 9）,15.54-155.40μg ？ ml^（-1）（r =0.999 8）,6.45-64.50μg ？ ml^（-1）（r = 0.9999） and 15.57-155.70μg ？ ml^（-1）（r = 0.999 9 ）.respectively.The average recovery was 100.80%,100.46%, 101.06%and 100.55%with RSD of 1.31%,1.72%.2.00%and 1.67%,respectively.Conclusion：The method is simple and accurate with good reproducibility and can be used in the quality control of Ganfukang pills.

关 键 词：高效液相色谱法 五味子醇甲 五味子酯甲 五味子甲素 五味子乙素 肝复康丸 

分 类 号：R927.2[医药卫生—药学]