HPLC法同时测定金鸡片中盐酸巴马汀和盐酸小檗碱的含量  

Simultaneous Determination of Palmatine Hydrochloride and Berberine Hydrochloride in Jinji Tablets by HPLC

作　　者：胡安青 

机构地区：[1]镇江市药品检验所,江苏镇江212003

出　　处：《中国药师》2012年第11期1590-1592,共3页China Pharmacist

摘　　要：目的:建立同时测定金鸡片中盐酸巴马汀和盐酸小檗碱含量的高效液相色谱法。方法:采用Agilent Zorbax SB-C_(18)柱(250 mm×4.6 mm,5μm);流动相:乙腈-0.05 mol·L^(-1)磷酸二氢钾溶液(用磷酸调节pH至3.0)(30:70),流速为1.0 ml·min^(-1),检测波长为265 nm。结果:盐酸巴马汀和盐酸小檗碱峰均能良好分离,进样量的线性范围分别为3.28～32.75μg·ml^(-1)和3.10～30.98μg·ml^(-1),平均加样回收率分别为99.4%和99.4%,RSD分别为0.9%和0.6%(n=6)。结论:所用方法简便,准确,重复性好,可用于金鸡片的含量测定。Objective：To establish an HPLC method for the simultaneous determination of paimatine hydrochloride and berberine hydrochloride in Jinji tablets.Method：An Agilent Zorbax SB-C_（18） column（250 mm×4.6 mm,5μm） was used.The mobile phase consisted of acetonitrile-0.05 mol ？ L^（-1） potassium dihydrogen phosphate（adjusting pH to 3.0 by phosphoric acid）（30：70）.The flow rate was 1 ml ？ min^（-1） and the detection wavelength was at 265 nm.Result;The content of paimatine hydrochloride and berberine hydrochloride was linear over the range of 3.28-32.75μg ？ ml^（-1） and 3.10-30.98μg ？ ml^（-1）,respectively.The average recovery was 99.4%and 99.4%with RSD of 0.9%and 0.6%（n=6）,respectively.Conclusion：The method is simple,accurate,reproducible and can be used in the content determination of Jinji tablets.

关 键 词：金鸡片 盐酸巴马汀 盐酸小檗碱 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]