壳寡糖对卵巢癌SKOV-3细胞生长抑制和凋亡诱导研究  

Effect of Chitosan Oligosacharide on the Inhibition and Apoptosis of Ovaian Carcinoma SKOV-3 Cells

作　　者：高宗华[1] 吕耀凤[1] 霍冠华[1] 

机构地区：[1]滨州医学院,山东烟台264003

出　　处：《中药材》2012年第10期1633-1636,共4页Journal of Chinese Medicinal Materials

基　　金：山东省自然科学基金(Y2006C01);山东省教育厅科技计划(J08LG15);烟台市科技发展计划(2008162)

摘　　要：目的:研究壳寡糖对卵巢癌生长增殖抑制和凋亡诱导作用,并探讨其作用机理。方法:不同浓度壳寡糖处理卵巢癌细胞,用倒置荧光显微镜、流式细胞仪、MTT等检测壳寡糖对卵巢癌细胞的抑制作用和凋亡诱导作用。结果:壳寡糖能有效抑制卵巢细胞SKOV-3的增殖,随着壳寡糖浓度增加,对SKOV-3细胞生长抑制率也会逐步增加;药物作用时间越长,细胞生长抑制率随之增加。Annexin V/PI双标记流式细胞术检测结果显示,壳寡糖处理SKOV-3细胞能明显诱导凋亡,并呈剂量依赖性。DNA凝胶电泳显示壳寡糖能诱导SKOV-3细胞凋亡。结论:壳寡糖能有效地抑制卵巢癌细胞增殖并明显诱导凋亡。Objective：To explore the effect and mechanism of inhibition and apoptosis of SKOV-3 cells induced by Chitosan oligosaccharide.Methods：Human ovarian cancer SKOV-3 cells were treated by various concentrations of Chitosan oligosaccharide.The inhibition of cell proliferation was tested by MTT.Apoptosis induced by Chitosan oligosaccharide was analysed by flow cytometry and agarose gel SKOV-3 cells DNA ladder electrophoresis.Results：Chitosan oligosaccharide could efficiently inhibit SKOV-3 cells proliferation in a dose-dependent manner.Also,the significantly apoptosis of SKOV-3 cells induced by Chitosan oligosaccharide was confirmed through flew cytometry with Annexin V/PI double labels and agarose gel DNA ladder electrophoresis.Conclusion：Chitosan oligosaccharide can efficiently inhibit SKOV-3 cells proliferation and induce its obviously apoptosis.

关 键 词：壳寡糖 卵巢癌 SKOV-3细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]