半夏泻心汤活性成分最佳组合筛选及其对胃溃疡大鼠Leptin、ET-1的影响  

Effect of Banxia Xiexin Decoction on Leptin and Endothelin-1 of Gastric Ulcer Rat and the Optimal Combination Screening of Active Components

作　　者：梁雪冰[1] 孙俊[2] 赵国平[1] 

机构地区：[1]暨南大学医学院,广东广州510632 [2]湖北省黄石市中心医院消化内科,湖北黄石435000

出　　处：《中药材》2012年第10期1637-1640,共4页Journal of Chinese Medicinal Materials

摘　　要：目的:筛选半夏泻心汤5种活性成分对胃溃疡模型大鼠的最佳组合,并观察其对Leptin、ET-1的影响。方法:SD大鼠87只随机分为正常对照组、假手术组、乙酸性胃溃疡组、奥美拉唑阳性对照组,并对半夏泻心汤中5种相关活性成分甘草次酸、β-谷甾醇、小檗碱、黄芩苷及人参总皂苷进行L16正交设计分组。用药后测定溃疡面积,Leptin和ET-1的及两者mRNA表达。结果:正交设计16组中,β-谷甾醇+小檗碱组的溃疡面积最小(P<0.05),甘草次酸+人参总皂苷组的血清Leptin含量最高(P<0.05);甘草次酸组的血浆ET-1含量均数最低(P<0.05)。与模型组比较,小檗碱组的胃组织Leptin mRNA表达水平升高最为显著(P<0.01);β-谷甾醇组胃组织ET-1 mRNA表达水平显著降低(P<0.01)。结论:半夏泻心汤相关活性成分可通过升高Leptin水平、降低ET-1水平起到促进胃溃疡愈合的作用。Objective：To select the optimal combination of five active component of Banxia Xiexin Decoction on gastric ulcer rat,and observe its effect on Leptin and ET-1.Methods：Eighty-seven SD rats were randomly divided into normal group,sham-operated group and acetic acid-induced gastric ulcer group,omeprazole group as a positive control,five active components（glycyrrhetic acid,β-sitosterol,berberine,baicalin and ginsenoside） of Banxia Xiexin Decoction were divided into groups by L16orthogonal design.The ulcer area,and the content of Leptin and ET-1,and the mRNA expression level of both were detected.Results：Among the sixteen orthogonal design groups,the ulcer area of these groups using both β-sitosterol and berberine was the smallest（P0.05）,the content of Leptin of these groups using both glycyrrhetic acid and ginsenoside was the highest in blood serum（P0.05）,the group using glycyrrhetic acid had the minimum concentration of ET-1 in blood plasma.Compared with model group,berberine could raise the mRNA expression level of Leptin（P0.01）,and β-sitosterol could lower the mRNA expression level of ET-1（P0.01）.Conclusion：The pathogenesis of gastric ulcer may be related with the down-regulation of concentration and mRNA expression level of Leptin,and upregulation of concentration and mRNA expression level of ET-1,the active components in Banxia Xiexin Decoction may upregulated Leptin and inhibit ET-1 to accelerate the healing of gastric ulcer.

关 键 词：半夏泻心汤活性成分 胃溃疡 正交设计 瘦素 内皮素-1 

分 类 号：R285.5[医药卫生—中药学]