黄瓜全基因组转录因子MADS-box家族基因的序列特征分析  

GENOME-WIDE SEQUENCE CHARACTERIZATION ANALYSIS OF MADS-BOX TRANSCRIPTION FACTOR GENE FAMILY IN CUCUMBER(Cucumis sativus L.)

作　　者：甘德芳[1] 丁飞[1] 庄丹[1] 梁丹迪[1] 

机构地区：[1]安徽农业大学园艺学院,安徽合肥230036

出　　处：《核农学报》2012年第9期1249-1256,共8页Journal of Nuclear Agricultural Sciences

基　　金：安徽省自然基金(1208085MC36);安徽省教育厅自然科学研究重点项目(KJ2011A110);安徽农业大学引进和稳定人才科研项目(wd2011-14)

摘　　要：MADS-box家族基因是一类编码转录因子的基因,因其在调控植物生长发育方面的重要功能而成为作物遗传育种研究的热点。采用生物信息学方法,对黄瓜全基因组MADS-box基因进行序列特征分析,包括CsMADS基因的数量、染色体定位、保守基序的分布及系统进化树分析。结果表明,黄瓜基因组中共有41个CsMADSs基因,除7个分布于Scaffold外,其他34个CsMADSs基因分布于黄瓜7条染色体上,但在染色体上的分布不是均匀的;41个CsMADSs都具有保守的MADS结构域,分布于9个MADS亚家族,而且大部分的CsMADSs基因属于SEP和MEF2-like分支。该研究结果不仅有助于MADS-box转录因子信号转导途径的解析,而且可为进一步研究该家族基因的功能提供信息参考。MADS-box is a large gene family which encodes transcription factors and plays important roles in the regulation of plant growth and development. Bioinformatics analysis of MADS-hox gene family in cucumber, including the number of the CsMADS genes, chromosomal localization, the distribution of conserved motifs and phylogenetic relationship were carried out. 41 CsMADS genes in cucumber were identified, 7 of which were in the scaffold while other 34 on 7 chromosomes. All the 41 CsMADSs had a conserved MADS domain. Based on the phylogenetic analysis of MADS-box protein sequences, 41 CsMADSs were categorized into 9 subfamilies, and most of which belonged to the SEP or MEF2-1ike branch. The results provide useful information for further functional analysis of MADS-box gene family in cucumber.

关 键 词：黄瓜 MADS—box 转录因子 生物信息学 

分 类 号：S642.2[农业科学—蔬菜学]