HPLC-MS法表征坝漆酚类化合物的结构  

Identification of Chemical Structure of Urushiol from Maoba Lacquer by HPLC-MS

作　　者：何源峰[1,2,3,4] 王成章[1,2,3,4,5] 陆榕[6] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家工程实验室 [3]国家林业局林产化学工程重点开放性实验室 [4]江苏省生物质能源与材料重点实验室,江苏南京210042 [5]中国林业科学研究院林业新技术研究所,北京100091 [6]明治大学

出　　处：《林产化学与工业》2012年第6期47-51,共5页Chemistry and Industry of Forest Products

基　　金：国际合作项目资助(S2012GR0138);国家林业局948技术引进项目(2012-4-05)

摘　　要：应用中低压柱分离毛坝漆酚类化合物,漆酚质量分数90%以上。应用HPLC、HPLC-MS、MS分析表征毛坝漆漆酚化学结构,HPLC以甲醇-水体积比9:1为流动相,总流速1 mL/min,UV 210 nm能够分离18个酚类化合物,HPLC-MS表征13个酚类化合物,主要为C15不饱和三烯漆酚和C17不同饱和度的漆酚。漆酚的中低压柱分离及HPLC跟踪为漆酚提供了快速分离方法。Middle pressure chromatography was used to purify urushiol. Its purity reached above 90%. HPLC, HPLC-MS, MS was applied to analyze urushiol that was extracted from Maoba lacquer. HPLC was performed under a methanol-water system（ volume ratio） with a flow rate of lmL/min. Eighteen peaks appeared under UV detector of 210 nm. Thirteen compounds were separated by HPLC-MS, mainly contained C15/17 with different degrees of unsaturation. The middle pressure chromatography and HPLC could be used as a efficient preparation and analysis method.

关 键 词：毛坝漆 漆酚 HPLC—MS 结构分析 

分 类 号：TQ35[化学工程]