头花蓼枯萎病的病原鉴定及室内毒力测定  

Pathogen Identification of Fusarium Wilt and Indoor Toxicity Determination of Polygonum capitatum

作　　者：苏凯[1] 桑维钧[1] 王慧[1] 杨尚华 潘滴滴 

机构地区：[1]贵州大学农学院贵州山地农业病虫害重点实验室,贵州贵阳550000 [2]贵州威门药业股份有限公司,贵州贵阳550025 [3]贵州省镇远县农业局,贵州镇远557700

出　　处：《贵州农业科学》2012年第12期95-98,共4页Guizhou Agricultural Sciences

基　　金：贵州省中药现代化产业专项子项目"苗药头花蓼病虫害无害化防治技术研究";"贵州省地道中药材病虫害调查及重要病虫防治技术研究"[黔科合中药专字(2003)52];贵州省农业科技攻关项目"黔北撑绿竹根茎腐烂病成因及防控技术研究"[黔科合字NY(2011)3050]

摘　　要：近年来贵州施秉县头花蓼发生一种枯萎病,该病害分布广、危害严重,已经成为影响当地头花蓼生产的潜在威胁。为了控制该病害的发生与危害,对头花蓼典型发病组织进行了病原菌分离鉴定和回接试验。结果表明,分离到的3种病原菌分别是弯孢属菌(Cochliobolus curvularia)、立枯丝核菌(Rhizoctoniasolani)和厚垣镰孢霉菌(Fusariumch lamydosporum)。经过接种试验,仅厚垣镰孢霉菌能引致与田间病害一致的症状,结合形态鉴定和ITS序列分析,初步确定该菌是头花蓼枯萎病的致病菌。室内抑菌试验表明,杀菌剂咪鲜胺、多菌灵、百菌清和恶霉灵对其有较好的抑制作用。In recent years Fusarium wilt had a wide distribution and severe damage to P. capitatum in Shibing County in Guizhou, which had already been the potential threaten to local P. capitatum production. In this paper the pathogen in typical diseased tissue of P. capitatum was isolated and identified and a tie-back test was conducted to control the occurance and damage. The results showed that the three isolated pathogens were Cochliobolus lamydosporum. The inoculation test showed that curvularia , Rhizoctonia solani and Fusariumch only F. lamydosporurn could cause symptom in accordance with field disease. After morphological identification and ITS sequence analysis, it was preliminarily concluded that F. lamydosporum was the pathogenic bacterium. Bacteriostasis test showed that prochloraz, carbendazim, chlorothalonil and hymexazol had preferable inhibitory action on F. lamydosporum.

关 键 词：头花蓼 厚垣镰孢霉 病原鉴定 毒力 

分 类 号：S431.18[农业科学—农业昆虫与害虫防治]