水田七的组织培养和植株再生  

Tissue Culture and Plant Regeneration of Schizocapsa plantaginea Hance

作　　者：张文珠[1,2] 林炳英[1,2] 林德钦 

机构地区：[1]厦门华侨亚热带植物引种园 [2]国家农作物国外引种隔离检疫基地,福建厦门361002

出　　处：《热带农业科学》2012年第12期19-22,共4页Chinese Journal of Tropical Agriculture

基　　金：厦门市科技创新资金计划项目(NO.3502Z20102006)

摘　　要：用水田七成熟种子为材料进行无菌播种,得到无菌苗再用幼叶、叶柄为材料进行组织培养和植株再生。经试验得出各阶段适宜的培养基分别为:(1)诱导愈伤组织:MS+2.0 mg/L TDZ;(2)诱导芽分化:MS+1.5 mg/L6-BA+0.5 mg/L NAA;(3)生根培养:1/2MS+0.5 mg/L IBA+0.1 mg/L NAA。The young leaves and leaf stalks of sterile seedlings were used as explants to study tissue culture and plant regeneration of Schizocapsa plantaginea Hance. The results showed the best medium of callus induction was solid Murashige and Skoog （MS） medium plus 2.0 mg/L TDZ, the highest frequency of shoot differentiation is obtained from the calli cultured on MS medium plus 1.5 mg/L 6-BA and 0.5 mg/L NAA, the best medium of rooting was 1/2 solid MS medium plus 0.5 mg/L IBA and 0.1 mg/L NAA.

关 键 词：水田七 愈伤组织 组织培养 植株再生 

分 类 号：S539[农业科学—作物学]