重组白细胞抑制因子和水蛭肽嵌合蛋白异构体鉴定  

Identification of Recombinant Leukocyte Inhibitory Factor and Hirulog Chimeric Protein Isomers

作　　者：刘巍[1] 范开[1] 

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054

出　　处：《重庆理工大学学报（自然科学）》2012年第12期47-53,共7页Journal of Chongqing University of Technology：Natural Science

摘　　要：提出了重组白细胞抑制因子和水蛭肽嵌合蛋白异构体鉴定检定方法。采用适宜的质控检测方法,通过试验RP-HPLC法、等电聚焦、非还原型SDS-PAGE凝胶电泳法、非变性PAGE法、活性分析、阴离子交换HPLC分析法对异构体进行定性和定量控制。结果表明:用建立的RP-HPLC法、非变性PAGE法、活性分析法不能鉴别异构体;等电聚焦、非还原型SDS-PAGE凝胶电泳法能鉴别异构体,可定性分析;阴离子交换HPLC分析法能对异构体百分含量进行定量分析。由此表明,等电聚焦、非还原型SDS-PAGE凝胶电泳和阴离子交换HPLC分析法可作为重组白细胞抑制因子和水蛭肽嵌合蛋白产品中异构体的常规检定。This paper established the recombinant leukocyte inhibition factor and leech peptide chimeric protein isoforms identification verification method,by using the appropriate quality control detection method,RP-HPLC method through the test,isoelectric focusing,a reduced SDS-PAGE gel electrophoresis,non-denaturing PAGE method,activity analysis,anion exchange HPLC analysis of isomers of qualitative and quantitative control.Using the established RP-HPLC method,PAGE method non degeneration,activity analysis examination could not identify isomers,and isoelectric focusing,a reduced SDS-PAGE gel electrophoresis to identify isomers,qualitative analysis.Anion exchange HPLC analyzed isomers＇ percentage quantitatively.Isoelectric focusing,a reduced SDS-PAGE gel electrophoresis and anion exchange HPLC analysis can be used as recombinant leukocyte inhibition factor and Hirudin Peptide chimeric protein products of isomer in routine test.

关 键 词：白细胞抑制因子 水蛭肽 异构体鉴定 

分 类 号：R972[医药卫生—药品]