HPLC法同时测定皱皮木瓜中原儿茶酸和绿原酸的含量  

Simultaneous determination of protocatechuic acid and chlorogcnic acid in Chaenomeles speciosa (Sweet) Nakai by HPLC

作　　者：李霞[1,2] 杨颖博[2] 李君丽[1] 陈程[1] 陈万生[2] 孙连娜[1] 

机构地区：[1]第二军医大学药学院,上海200433 [2]第二军医大学附属长征医院药学部,上海200403

出　　处：《中国医药导报》2012年第34期110-112,共3页China Medical Herald

基　　金：国家科技重大专项子课题-重大新药创制项目(项目编号:2011ZX09102-006-03)

摘　　要：目的建立皱皮木瓜中主要有效成分原儿茶酸和绿原酸的高效液相色谱含量测定方法。方法采用DikmaDiamonsil C18色谱柱(4.6 mm×250.0 mm,5μm),甲醇-0.1%甲酸水梯度洗脱,流速:1.0 mL/min,柱温:30℃,检测波长:254 nm。结果原儿茶酸在16.66～533.00μg(r=0.999 9),绿原酸在23.91～765.00μg(r=0.999 5),线性关系良好;两个化合物的平均回收率分别为100.11%(RSD=0.90%,n=6)及99.06%(RSD=0.52%,n=6)。结论该方法简便快速,分离效果好,灵敏度高,重现性好,可为全面控制皱皮木瓜质量提供参考。Objective To develop an HPLC method for the simultaneous quailtitation of protocatechuic acid and chlorogcnic acid in Chaenomeles speciosa（Sweet） Nakai.Methods Samples were analyzed on Dikma Diamonsil C18 column（4.6 mm× 250.0 mm,5 μm）,eluted with methanol and water containing 0.1% formic acid as mobile phases in a linear gradient mode.The flow rate was kept at 1.0 mL/min and the column temperature was set to 30 ℃,the detector wavelengths were 254 nm.Results The linear ranges were 16.66-533.00 μg（r=0.999 9） for protocatechuic acid,23.91-765.00 μg（r=0.999 5） for chlorogcnic acid.The average recoveries of the two constituents were 100.11%（RSD=0.90%,n=6）,99.06%（RSD=0.52%,n=6）,respectively.Conclusion The method is sensitive,accurate,repeatable,and can be used for quality control of the fruits of Chaenomeles speciosa（Sweet） Nakai.

关 键 词：木瓜药材 高效液相色谱法 含量测定 原儿茶酸 绿原酸 

分 类 号：O657.63[理学—分析化学]