巨噬细胞炎性蛋白-1α在子宫内膜异位症中的表达及意义  

Expression and significance of macrophage in ammatory protein 1α in endometriosis

作　　者：胡可可[1] 谭琛[1] 张琴[1] 陈晓琼[1] 邓赫男[1] 肖斌梅[1] 

机构地区：[1]湖南省郴州市第一人民医院妇产科,湖南郴州423000

出　　处：《中国当代医药》2012年第35期18-20,共3页China Modern Medicine

摘　　要：目的观察巨噬细胞炎性蛋白-1α(MIP-1α)在子宫内膜异位症中的表达,并探讨其临床意义。方法采用酶联免疫吸附法测定35例子宫内膜异位症患者(实验组,其中Ⅰ～Ⅱ期20例,Ⅲ～Ⅳ期15例)和25例行子宫切除术的子宫肌瘤患者(对照组)经血、血清、腹腔液MIP-1α浓度,通过RT-PCR检测两组子宫内膜组织中MIP-1αmRNA的表达。结果实验组Ⅰ～Ⅱ期、Ⅲ～Ⅳ期经血和腹腔液中MIP-1α浓度及子宫内膜组织MIP-1αmRNA表达水平显著高于对照组(P<0.01或P<0.05),且Ⅲ～Ⅳ期高于Ⅰ～Ⅱ期(P<0.01)。两组血清MIP-1α浓度比较,差异无统计学意义(P>0.05)。结论 MIP-1α可能在子宫内膜异位症的发病机制中起重要作用,经血中MIP-1α的检测具有方便、无创、价廉、依从性好等优点,可望成为诊断子宫内膜异位症的生物学指标。Objective To observe the expression of macrophage in ammatory protein 1α（MIP-1α） in endometriosis,and explore its clinical significance.Methods The concentration of MIP-1α in menstrual effluent,serum and peritoneal fluid was detected using enzyme-linked immunosobent assay from 35 EMs patients（experimental group） including 20 women withⅠ-Ⅱ stages and 15 women with Ⅲ-Ⅳ stages and 25 patients with uterine fibroids suffering hysterectomy（control group）.Expression levels of MIP-1α mRNA were measured by RT-PCR in endometrium.Results MIP-1α concentrations in menstrual effluent and peritoneal and expression levels of MIP-1α mRNA in experimental group were significantly increased in Ⅰ-Ⅱand Ⅲ-Ⅳ stages compared with control group（P 〈0.01 or P 〈0.05）,furthermore these indicators were higher in Ⅲ-Ⅳstages thanⅠ-Ⅱ stages（P 〈0.01）.There was no significant difference in serum MIP-1α concentration between experimental group and control group（P 〈0.05）.Conclusion MIP-1α may play an important role in the pathogenesis of endometriosis.Determination of MIP-1α in menstrual effluent is convenient,cheap,non-invasive and possesses good compliance,which might be a valuable index for the diagnosis of endometriosis.

关 键 词：巨噬细胞炎性蛋白-1Α 子宫内膜异位症 表达 意义 

分 类 号：R711.71[医药卫生—妇产科学]