罗格列酮对兔早期动脉粥样硬化白细胞介素-6、白细胞介素-8表达的影响  

Effects of rosiglitazone on interleukin-6 and interleukin-8 expressions in rabbits with early arteriosclerosis

作　　者：贾军正[1] 李万玲[1] 郭文芬[1] 王志禄[2] 徐芳[1] 蒙颖[1] 

机构地区：[1]兰州大学第一临床医学院,730000 [2]兰州大学第一医院心内科,730000

出　　处：《国际心血管病杂志》2012年第6期372-376,共5页International Journal of Cardiovascular Disease

基　　金：甘肃省自然科学研究基金资助(1010RJZA122);甘肃省卫生行业科研计划管理项目(GWGL2010-16)

摘　　要：目的:比较罗格列酮和辛伐他汀对兔早期动脉粥样硬化白细胞介素(IL)-6、IL-8表达的影响,探讨罗格列酮抗动脉粥样硬化的作用机制。方法:将36只雄性新西兰兔随机分为对照组、模型组、辛伐他汀组和罗格列酮组。采用皮下注射同型半胱氨酸硫内酯(HTL)及高脂饲养结合的方法建立兔动脉粥样硬化模型,酶联免疫吸附法(ELISA)检测血清中IL-6和IL-8的水平,免疫组化检测兔主动脉粥样硬化组织中IL-6和IL-8的表达。结果:与对照组相比,其他3组血清及病变组织中IL-6、IL-8表达均显著升高;与模型组比较,辛伐他汀和罗格列酮组IL-6、IL-8表达显著降低;罗格列酮组IL-6、IL-8表达较辛伐他汀组更低(P均<0.05)。结论:罗格列酮能通过抑制兔主动脉粥样硬化组织及血循环中IL-6、IL-8等炎症因子的表达,发挥其抗炎作用,拮抗动脉粥样硬化的发生及发展。Objective：To compare the effects of rosiglitazone and simvastatin on the expressions of interleukin （IL-6 and interleukin （IL-8 in atherosclerotie rabbit model and to explore the anti atherosclerotic properties of rosiglitazone. Methods： A total of 36 male New Zealand rabbits were randomly divided into control group, model group, simvastatin group and rosiglitazone group. Subcutaneous injection of dl-homocysteine thiolactone （HTL） with cholesterol enriched diet was used to reproduce atherosclerotic rabbit model. Serum IL-6 and IL 8 were examined at the method of enzyme- linked immunosorbent assay （ELISA） at the end of 10 weeks. Immunohistochemistry staining was performed to observe IL- 6 and IL-8 protein expressions in each group. Results： The experimental results both in the serum and in the diseased region have shown that compared with the control group, the IL-6 and IL-8 expressions were significantly increased in the other three groups （P ~ 0. 05）. Compared with the model group, the expressions of IL-6 and IL 8 in the simvastatin group and the rosiglitazone group were decreased significantly （P〈0. 05）. The expressions of IL-6 and IL-8 in the rosiglitazone group were decreased significantly when compared with the simvastatin group （P〈0.05）. Conclusion：Rosiglitazone may reduce the expression of the inflammatory factors such as IL-6 and IL-8 both in the serum and in the diseased region, and alleviate the inflammatory reaction contributing to atherosclerosis.

关 键 词：罗格列酮 辛伐他汀 动脉粥样硬化 白细胞介素-6 白细胞介素-8 

分 类 号：R965[医药卫生—药理学]