HPLC法同时测定肺闭宁颗粒中麻黄碱与伪麻黄碱含量  

Determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Feibining granule by HPLC

作　　者：孙蓓[1] 张蓓[2] 陈双璐[3] 

机构地区：[1]天津医科大学附属肿瘤医院门诊办公室天津市肿瘤防治重点实验室,天津300060 [2]天津市第一中心医院药剂科,天津300192 [3]天津市儿童医院药剂科,天津300074

出　　处：《天津医科大学学报》2012年第4期507-509,共3页Journal of Tianjin Medical University

摘　　要：目的:建立HPLC法同时测定肺闭宁颗粒中盐酸麻黄碱与盐酸伪麻黄碱含量的方法。方法:色谱柱为Shim-pack VP-ODS C18柱(150 mm×4.6 mm,5μm);流动相为乙腈-水-磷酸-三乙胺(3∶97∶0.1∶0.2);流速为1.0 mL/min;检测波长为213 nm。结果:盐酸麻黄碱在0.456～1.596μg、盐酸伪麻黄碱在0.356～1.246μg的浓度范围内与峰面积呈良好的线性关系,平均回收率为:盐酸麻黄碱99.49%,RSD为1.73%;盐酸伪麻黄碱98.56%,RSD为1.12%(n=6)。结论:该方法简便,结果准确,重现性好,可作为该制剂的质量控制方法。Objective： To establish an HPLC method for determination of ephedrine hydroehloride and pseudoephedrine hydrochloride in Feibining granule. Methods： RP-HPLC method was used with Shim-pack VP-ODS C18 column （150 mm×4.6 mm, 5 μm）. The mobile phase was consisted of acetonitrile-water-H3_PO4triethylamine （3：97：0.1：0.2）. The flow rate was 1.0 mL·min-1. The detection wawe- length was at 213 nm. Results： The linear range of ephedrine hydrochloride was 0.456-1.596 μg. The average recovery was 99.49%, and RSD was 1.73%. The linear range of pesudoephedrine hydrochloride was 0.356- 1.246 μg;The average recovery was 98. 56%, and RSD was 1.12%. Conclusion： This method is simple. The result is accurate and the reproducibility is good. Theremothods can be used as the quality control of Feibining granules.

关 键 词：肺闭宁颗粒 盐酸麻黄碱 盐酸伪麻黄碱 HPLC法 质量控制 

分 类 号：R927.2[医药卫生—药学]