软坚护肝片对四氯化碳所致小鼠急性肝损伤的保护作用  

Protective effect of Ruanjian hugan tablet on acute hepatic injury induced by carbon tetrachloride in mice

作　　者：莫凤珍[1] 孔晓龙[1] 覃振明[1] 汪永玲[1] 梁凯[1] 黄绍德[1] 

机构地区：[1]广西医科大学药学院药理学教研室,南宁530021

出　　处：《中国临床新医学》2012年第12期1104-1107,共4页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：广西科学技术开发计划项目(编号:桂科攻10124008-7)

摘　　要：目的观察软坚护肝片(RJHGT)对四氯化碳(CCl4)所致小鼠急性肝损伤的保护作用,并探讨其作用机制。方法腹腔注射CCl4制备小鼠急性肝损伤模型,检测血清谷丙转氨酶(AST)、谷草转氨酶(ALT)、碱性磷酸酶(AKP)活性及总抗氧化能力(T-AOC);检测肝组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)活性。结果 RJHGT各组能够减轻肝细胞损伤程度,与模型组比较,RJHGT能降低血清ALT、AST、AKP的活性及肝组织中MDA、GSH的含量(P<0.01或<0.05);提高T-AOC能力及SOD、GSH-PX的活力(P<0.01或<0.05)。结论 RJHGT对CCl4所致小鼠急性肝损伤具有明显的保护作用,其机制可能与其抗脂质过氧化作用有关。Objective To study the protective effect of Ruanjian hugan tablet（RJHGT） on acute hepatic in- jury induced by carbon tetrachloride （ CC14 ） in mice and its mechanism. Methods CC14 was given by intrapcritoneal injection to establish acute hepatic injury model. Thereafter, blood was detected the activities of ALT, AST, AKP and T-AOC in serum; Liver homogenate was measured the content of MDA, GSH and the activities of SOD, GSH- PX. Results RJHGT can lessened the degree of hepatic injury. Compared with the model group, the activities of AST, ALT,AKP and the content of MDA in the treatment groups were decreased; while the ability of T-AOC, the content of GSH and the activity of SOD and GSH-PX were increased （ P 〈 0.01 or P 〈 0. 05 ）. Conclusion RJHGT has protective effect on acute liver injury induced chemically. The mechanism may be related to inhibiting the effect on lipid peroxidation.

关 键 词：软坚护肝片 四氯化碳 急性肝损伤 

分 类 号：R285.5[医药卫生—中药学]