New chiral reverse phase HPLC method for enantioselective analysis of ketorolac using chiral AGP column  

作　　者：Sunil K. Dubey Jangala Hemanth Chiranjeevi Venkatesh K. R.N. Saha S. Pasha 

机构地区：[1]Department of Pharmacy, Birla Institute of Technology & Science, Pilani, India [2]Institute of Genomics and Integrative Biology, Mall Road, Delhi, India

出　　处：《Journal of Pharmaceutical Analysis》2012年第6期462-465,共4页药物分析学报（英文版）

基　　金：University Grant Commission,New Delhi for extending their financial support

摘　　要：A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer （pH 4.5）： lsopropanol （98：2, v/v）, at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detection was made at 322 nm. The linearity range was 0.02 10 μg/mL for each of the enantiomers. The mobile phase composition was systematically studied to find the optimum chromatographic conditions. Validation of the method under the conditions selected showed that it was selective and precise and that the detector response was linear function of ketorolac.A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer （pH 4.5）： lsopropanol （98：2, v/v）, at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detection was made at 322 nm. The linearity range was 0.02 10 μg/mL for each of the enantiomers. The mobile phase composition was systematically studied to find the optimum chromatographic conditions. Validation of the method under the conditions selected showed that it was selective and precise and that the detector response was linear function of ketorolac.

关 键 词：R-KetorolacS-Ketorolac ChiralAGP RP-HPLC 

分 类 号：R927[医药卫生—药学]