丹参酮-ⅡA磺酸钠对脊髓缺血再灌注损伤NF-κB、VCAM-1和血液流变性的影响  

Effects of Tanshinone-ⅡA sulfonate on expression of Nuclear factor kappaB,Vascular Cell Adhesion Molecule-1 and hemorrheology during spinal cord ischemia reperfusion injury

作　　者：张俐[1] 安国尧[1] 张文光[1] 陈凯[1] 

机构地区：[1]福建中医药大学骨伤学院省部共建教育部重点实验室,福建福州350108

出　　处：《中国骨伤》2012年第12期1016-1020,共5页China Journal of Orthopaedics and Traumatology

基　　金：国家自然科学基金(编号:30973765);国家新世纪人才支持计划(编号:NECT-09-0013);教育部博士点基金(编号:20113519110001)~~

摘　　要：目的:观察丹参酮-ⅡA磺酸钠对脊髓缺血再灌注损伤关键因子核因子-κB(nuclear factor-kappaB,NF-κB)、血管细胞粘附分子-1(vascular cell adhesion molecule-1,VCAM-1)及血液流变性的影响,探讨该药对脊髓缺血再灌注损伤的作用及机制。方法:选取3月龄新西兰家兔54只,体重为(2.0±0.2)kg,随机分为假手术组6只(术中仅分离腰动脉并于手术前后各0.5h沿耳缘静脉注射生理盐水,0.8ml/kg)、缺血组24只(分离后夹闭腰动脉,同上注射等剂量生理盐水)、丹参酮组24只(分离后夹闭腰动脉,同上注射等剂量丹参酮-ⅡA磺酸钠)。分别于给药后0.5、1、4、8h腹主动脉采血,用于检测全血粘度[高切(mpa.s)/150(l/s)、中切(mpa.s)/60(l/s)、低切(mpa.s)/10(l/s)]、毛细管血浆粘度、红细胞聚集指数、刚性指数、变形指数和电泳指数。同时取脊髓组织,分为两段,一段固定于4%的多聚甲醛中,另一段放入液氮中冻存,用免疫组化法和ELISA法检测3组各时点脊髓NF-κB、VCAM-1含量的变化。结果:①脊髓缺血再灌注损伤后NF-κB表达,同一时点免疫组化图片中丹参酮组表达最少,缺血组中表达最高。②与假手术组相比,缺血组各时点VCAM-1表达显著升高,在0.5、1、4h时差异显著(P<0.01),8h时具有差异性(P<0.05);此外,丹参酮组与缺血组相比,0.5h显著降低(P<0.01),1、4、8h时降低且具有差异性(P<0.05)。③假手术组始终无阳性血管表达,0.5h缺血组、丹参酮组亦无阳性血管表达;1、4、8h缺血组阳性血管表达率最高,其中1、4h中丹参酮组与缺血组相比较差异显著(P<0.05),8h差异显著(P<0.01)。④缺血组各时点全血粘度在10s-1、60s-1、150s-1升高,毛细管血浆粘度升高(P<0.05或P<0.01)。丹参酮组各时间点毛细管血浆粘度、红细胞聚集率、细胞刚性指数、变形指数降低极其显著(P<0.01)。结论:丹参酮-ⅡA磺酸钠减轻脊髓缺血再灌注损伤可能是通过调控炎性因子NF-КB、VCAM-1Objective：To observe effects of Tanshinone-Ⅱ A sulfonate on expression of Nuclear factor kappaB（NFκB）,Vascular Cell Adhesion Molecule-l（VCAM-1）and hemorrheology during spinal cord ischemia reperfusion injury,and explore the function and mechnism.Methods：Fifty-four New Zealand rabbits（aged 3 months,weighted 2.0±0.2 kg） were randomly divided into 6 in sham group（lumbar artery were separated in operation,0.8 ml /kg saline were injected at 0.5 h before and after operation）,24 in ischemia group（lumbar artery were clipped after seperation,and the same dose of saline）,24 in Tanshinone group（lumbar artery were clipped after seperation,and the same dose of Tanshinone-ⅡA sulfonate）.Abdomincal aorta blood were drawed after treatment respectively at 0.5 h,1 h,4 h and 8 h,and tesetd whole blood viscosity [high cut（mpa.s） /150（l /s）,middle cut（mpa.s） /60（l /s） and low cut（mpa.s） /10（l /s）],capillary plasma viscosity,red cell aggregation index,rigid index,deformation index and electrophoresis index.Spinal cord tissues were divided into two sections,one fixed in 4% paraformaldehyde,another stored in liquid nitrogen.Immunohistochemical method and ELISA were used to test change of content of NF-κB and VCAM-1.Results：①The expression of NF-κB in Tanshinone group were lowest,and in ischemia group were highest.②Compared with sham group,VCAM-1 in ischemia group at different time were obviously increased,especially at 0.5,1 and 4 h（P〈0.01）,and had meaning at 8 h（P〈0.05）.Compare between Tanshinone group and ischemia group,VCAM-1 at 0.5 h were obviously decreased（P〈0.01）,and had meaning at 1 h,4 h and 8 h（P〈0.05）.③There were no postive vasvular expression in sham group,and at 0.5 h in Tanshinone group and ischemia group.The highest postive vasvular expression in ischemia group were at 1 h,4 h and 8 h,and had significant meaning at 1 h and 4 h between ischemia group and Tanshinone group（P〈0.05）,and 8 h were obviously most.④The whole blood vi

关 键 词：脊髓缺血 脊髓损伤 血管细胞粘附分子-1 血液流变学 

分 类 号：R971.2[医药卫生—药品]