银屑病患者皮损中细胞凋亡情况的评价  

Evaluation of Cell Apoptosis in Lesional and Non- lesional Psoriatic Skin

作　　者：茅晓红[1] 程浩[1] 孙国均[1] 周凯[1] 陈智[2] 

机构地区：[1]浙江大学医学院附属第二医院皮肤科,杭州310009 [2]浙江大学医学院传染病研究所

出　　处：《中华皮肤科杂志》2000年第2期77-79,共3页Chinese Journal of Dermatology

基　　金：浙江省教委科研项目

摘　　要：目的 探讨细胞凋亡与银屑病的关系。方法 用免疫组化 SP法检测银屑病皮损与非皮损中 Bax、 Fas、 Fas－ L的表达，并用 TUNEL（末端脱氧核苷酰转移酶介导的 d－ UTP－生物素缺口末端标记）技术检测细胞凋亡情况。 结果 与正常人相比，银屑病表皮角质形成细胞，真皮炎症细胞及血管内皮均存在不同程度的上述蛋白的过度表达，伴细胞凋亡增多，部分在治疗后未完全恢复正常。 结论 推测银屑病皮损中角质形成细胞凋亡增多是针对细胞过度增殖的一种内在稳态机制，可能与 Bax , Fas及 Fas－ L等凋亡相关分子的异常表达有关，而 Fas系统还可能参与银屑病的局部免疫异常。Objective To study the relationship between cell apoptosis and psoriasis. Methods The expression of Bax, Fas and Fas- L was studied with immunohistochemical technique(SP), and apoptosis was detected with terminal deoxynucleotidyl transferase- mediated dUTP- biotin nick end labeling (TUNEL) assay in lesional and non- lesional psoriatic skin.Results In comparison with normal skin, there was an over- expression of Bax, Fas and Fas- L in epidermal keratinocytes, inflammatory cells and vascular endothelium in psoriatic skin, and accompanied with up- regulation of apoptosis. The above abnormalities were not corrected completely after effective treatment in some cases. Conclusions The increase of apoptotic keratinocytes is possibly one kind of internal homeostatic mechanism against the hyperplasia of epidermal cells, and might relate to abnormal expression of apoptosis- associated proteins Bax, Fas and Fas- L. The Fas system might also take part in unusual local immunoreaction in psoriasis.

关 键 词：银屑病 Bax FAS CD95抗原 FAS-L 细胞凋亡 

分 类 号：R758.6[医药卫生—皮肤病学与性病学]