Expression of inhibin α,matrix metalloproteinase-2 and tissue inhibitors of metalloproteinase-1 in luteinized granulosa cells:comparison between women with and without endometriosis  

作　　者：谭容容 刘丽鹏 浦丹华 崔毓桂 江世文 刘嘉茵 吴洁 

机构地区：[1]State Key Laboratory of Reproductive Medicine,Department of Obstetrics & Gynecology,the First Affiliated Hospital of Nanjing Medical University [2]Nanjing Medical University [3]Department of Biomedical Sciences,Mercer University School of Medicine,Savannah,GA 31405,USA [4]State Key Laboratory of Reproductive Medicine

出　　处：《生殖医学杂志》2012年第B12期61-69,共9页Journal of Reproductive Medicine

基　　金：supported by the Medical Research Council[grant number No.XK02 200904]

摘　　要：Objective:To assess the differences in expression of inhibin a,MMP-2 and TIMP-1 in luteinized granulosa cells from women with and without endometriosis undergoing in vitro fertilization(IVF),and determine whether inhibin a,MMP-2 and TIMP-1 expressions correlate with the follicle development in patients with endometriosis. Methods:Thirty six infertile patients with stageⅢandⅣendometriosis patients(group A) and 35 tubal infertile and/or male factor patients(group B) attending to the clinic of Jiangsu Provincial Hospital Human Reproductive Center during May 2011 and December 2011 were included in this prospective study.The patients with the number of mature oocyte≤4 were considered as poor responders.According to this,the two groups were further divided into four subgroups:Al group(23 endometriosis patients with normal response),A2 group(13 endometriosis patients with poor response),Bl group(23 controls with normal response) and B2 group(12 controls with poor response).Fasting blood samples were collected on the first day of ovarian stimulation and on the day of hCG administration,and follicle fluid were collected on the day of oocytes retrieved.The concentrations of follicle stimulating hormone(FSH),Luteinizing hormone(LH) and estradiol(E_2) were measured by ECILA.Granulosa was collected from follicle fluid by Percoll method,and Stepone real-time polymerase chain reaction(PCR) was used to analysis the expression level of inhibinα,MMP-2 and TIMP-1. Results;The expression of inhibinαin endometriosis group and poor responders in control group were significantly lower than those of the normal responders in control group,in which the alternation of inhibinαexpression was not correlated to the variables of follicle development.Moreover,MMP-2 levels were lower and TIMP-1 levels were higher in both endometriosis and tubal/male infertility poor responders compared to normal ovarian responders.Interestingly,the correlation between MMP-2 or TIMP-1 expression and variables of follicle development in tubal or male infeObjective： To assess the differences in expression of inhibin a, MMP-2 and TIMP-1 in luteinized granulosa cells from women with and without endometriosis undergoing in vitro fertilization （IVF）, and determine whether inhibin α, MMP-2 and TIMP-1 expressions correlate with the follicle development in patients with endometriosis. Methods： Thirty six infertile patients with stage III and IV endometriosis patients （group A） and 35 tubal infertile and/or male factor patients （group B） attending to the clinic of Jiangsu Provincial Hospital Human Reproductive Center during May 2011 and December 2011 were included in this prospective study. The patients with the number of mature oocyte 44 were considered as poor responders. According to this, the two groups were further divided into four subgroups： A1 group （23 endometriosis patients with normal response）, A2 group （13 endometriosis patients with poor response）, B1 group （23 controls with normal response） and B2 group （12 controls with poor response）. Fasting blood samples were collected on the first day of ovarian stimulation and on the day of hCG administration, and follicle fluid were collected on the day of oocytes retrieved. The concentrations of follicle stimulating hormone （FSH）, Luteinizing hormone （LH） and estradiol （Ez） were measured by ECILA. Granulosa was collected from follicle fluid by Percoll method, and Stepone real-time polymerase chain reaction （PCR） was used to analysis the expression level of inhibin a, MMP-2 and TIMP-1. Results： The expression of inhibin a in endometriosis group and poor responders in control group were significantly lower than those of the normal responders in control group, in which the alternation of inhibin a expression was not correlated to the variables of follicle development. Moreover, MMP-2 levels were lower and TIMP-1 levels were higher in both endometriosis and tubal/male infertility poor responders compared to normal ovarian responders. Interestingly, the correlation b

关 键 词：子宫内膜异位症 基质金属蛋白酶-2 颗粒细胞 抑制素 卵巢 聚合酶链反应 TIMP-1 抑制剂 

分 类 号：Q579.13[生物学—生物化学] Q257